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202029

Sigma-Aldrich

Metanil Yellow

Dye content 70 %

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Synonym(s):
3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36
Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
587-98-4
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein:
3831568
EC Number:
209-608-2
MDL number:
MFCD00007486
UNSPSC Code:
12171500
PubChem Substance ID:
24852034
NACRES:
NA.47

form

solid

Quality Level

200

composition

Dye content, 70%

technique(s)

titration: suitable

pH

1.5-2.7, red to yellow

λmax

414 nm

ε (extinction coefficient)

≥18500 at 415.0-419.0 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast. Metanil yellow changes its color from red at pH 1.2 to yellow at pH 2.3.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cellular Localization of Latent Murine Cytomegalovirus
Koffron A J, et.al.
Journal of Virology, 72, 95-103 (1998)
An Improved Stain for Connective Tissue Cells in Plastic-Embedded Sections
Cannon M S
Laboratory Medicine, 10, 783?785-783?785 (1979)
Photocatalytic degradation of azo dye Metanil Yellow: Optimization and kinetic modeling using a chemometric approach
Sleiman M
Applied Catalysis. B, Environmental, 77, 1-2 (2007)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et.al.
Biotechnic & Histochemistry, 66, 169-172 (1991)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et al.
Biotechnic & Histochemistry, 66, 169-169 (1991)
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