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201677

Sigma-Aldrich

Janus Green B

certified by the Biological Stain Commission, Dye content 65 %

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Synonym(s):
3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B
Empirical Formula (Hill Notation):
C30H31ClN6
CAS Number:
2869-83-2
Molecular Weight:
511.06
Colour Index Number:
11050
EC Number:
220-695-6
MDL number:
MFCD00011758
UNSPSC Code:
12171500
PubChem Substance ID:
24852018
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, 65%

color

black
very dark brown
very dark green

λmax

395 nm
660 nm (2nd)

ε (extinction coefficient)

≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C

InChI

1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1

InChI key

XXACTDWGHQXLGW-UHFFFAOYSA-M

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Application

Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.

Biochem/physiol Actions

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David E. Sadava
Cell Biology: Organelle Structure and Function (1993)
Intracellular insulin quantification by cell-ELISA
Andersen P and Vermette P
Experimental Cell Research, 347, 14-23 (2016)
Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method
Lee BC
Anatomical Record. Part B, New Anatomist, 286, 1-7 (2005)
R. W. Sabnis
Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications (2010)
Telocytes in trachea and lungs
Zheng Y
Journal of Cellular and Molecular Medicine, 15, 2262-2268 (2011)
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