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200964

Sigma-Aldrich

Erythrosin B

Dye content ≥95 %

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Synonym(s):
Solvent Red 140
Empirical Formula (Hill Notation):
C20H8I4O5
CAS Number:
15905-32-5
Molecular Weight:
835.89
Colour Index Number:
45430:2
EC Number:
240-046-0
MDL number:
MFCD00005044
UNSPSC Code:
12171500
PubChem Substance ID:
24851985
NACRES:
NA.47

form

powder

Quality Level

100

composition

Dye content, ≥95%

color

orange to red-orange

mp

303 °C (dec.) (lit.)

solubility

1 M NH4OH: 1 mg/mL
1 M NH4OH: soluble 1 mg/mL, orange to red

ε (extinction coefficient)

≥102000 at 526-534 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥13000 at 310-318 nm in 1 M NH4OH at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥32000 at 258-266 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(I)cc2c(Oc3c(I)c(O)c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

OALHHIHQOFIMEF-UHFFFAOYSA-N

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Application

Erythrosin B has been used as a dye in fluorescence lifetime imaging.

Biochem/physiol Actions

Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bright monomeric red fluorescent protein with an extended
fluorescence lifetime
Merzlyak EM
Nature Methods, 4, 555-557 (2007)
Practical and reliable FRET/FLIM pair of fluorescent proteins.
Shcherbo D
BMC biotechnology, 2009-2009 null
Erythrosin B is a specific inhibitor of high affinity 3H-ouabain binding and ion transport in rat brain
Silbergeld E K
Neuropharmacology, 20, 87-90 (1981)
Zhen Cheng et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 201, 281-287 (2018-05-15)
Because of the serious harm to animals and the environment associated with perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), a rapid, sensitive and low-cost method for detecting PFOS and PFOA is of great importance. In this paper, a novel sensing
Erythrosin B inhibits dopamine transport in rat caudate synaptosomes.
Lafferman JA and Silbergeld EK
Science, 205, :410-:412 (1979)
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