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200182

Sigma-Aldrich

Nitrazine Yellow

indicator grade, Dye content 85 %

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Synonym(s):
2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow
Empirical Formula (Hill Notation):
C16H8N4Na2O11S2
CAS Number:
5423-07-4
Molecular Weight:
542.36
Colour Index Number:
14890
EC Number:
226-549-8
MDL number:
MFCD00003938
UNSPSC Code:
12171500
PubChem Substance ID:
24851961
NACRES:
NA.47

grade

indicator grade

Assay

>90.0% (HPLC)

form

powder

composition

Dye content, 85%

solubility

H2O: 1 mg/mL

λmax

586 nm

ε (extinction coefficient)

≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;

InChI key

XBLIFEQTVVSTIM-QIKYXUGXSA-L

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Application

Nitrazine Yellow has been used in dye-protein binding assay.

Biochem/physiol Actions

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye. It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy. Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S L Bennett et al.
American journal of perinatology, 10(2), 101-104 (1993-03-01)
Most studies investigating the usefulness of ferning and nitrazine methods for detecting the presence of amniotic fluid following ruptured amniotic membranes have employed samples from vaginal pooling. Reported accuracies are 84 to 100% for the ferning test and 87 to
T Kishida et al.
European journal of obstetrics, gynecology, and reproductive biology, 58(1), 67-72 (1995-01-01)
We have developed a new anti-AFP monoclonal antibody kit which is easier to use than other examination methods for detecting AFP in the leaked amniotic fluid. In this study, we investigate the clinical value of this test in the diagnosis
Structural characterisation of Nitrazine Yellow by NMR spectroscopy
Viscardi G
Dyes and Pigments, 27, 87- 95 (2003)
Gilbert G G Donders et al.
Infectious diseases in obstetrics and gynecology, 2010, 504972-504972 (2010-10-19)
Accessing vaginal pH is fundamental during gynaecological visit for the detection of abnormal vaginal flora (AVF), but use of pH strips may be time-consuming and difficult to interpret. The aim of this study was to evaluate the VS-SENSE test (Common
The influence of a polyamide matrix on the halochromic behaviour of the pH-sensitive azo dye Nitrazine Yellow
Schueren L V D
Dyes and Pigments, 94, 443-451 (2012)
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