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Merck
CN

200182

Nitrazine Yellow

indicator grade, Dye content 85 %

Synonym(s):

2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow

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About This Item

Empirical Formula (Hill Notation):
C16H8N4Na2O11S2
CAS Number:
5423-07-4
Molecular Weight:
542.36
NACRES:
NA.47
PubChem Substance ID:
24851961
UNSPSC Code:
12171500
EC Number:
226-549-8
MDL number:
MFCD00003938

Key Documents

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form

powder

InChI key

XBLIFEQTVVSTIM-QIKYXUGXSA-L

InChI

1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;

SMILES string

[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O

grade

indicator grade

assay

>90.0% (HPLC)

composition

Dye content, 85%

solubility

H2O: 1 mg/mL

λmax

586 nm

ε (extinction coefficient)

≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Application

Nitrazine Yellow has been used in dye-protein binding assay.

Biochem/physiol Actions

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye. It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy. Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCD1402
MKBQ6886V
MKBG8083V
MKBD9527V
36296KJ

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Lien Van der Schueren et al.
Carbohydrate polymers, 91(1), 284-293 (2012-10-10)
Nanofibres functionalised with pH-sensitive dyes could greatly contribute to the development of stimuli-responsive materials. However, the application of biocompatible polymers is vital to allow for their use in (bio)medical applications. Therefore, this paper focuses on the development and characterisation of
Ibrahim A Abdelazim
Archives of gynecology and obstetrics, 287(2), 205-210 (2012-09-13)
This study was designed to detect the accuracy of the fetal fibronectin (Quick Check fFN test(®)) in diagnosing premature rupture of fetal membranes. This comparative prospective study was carried out over 1 year in Ahmadi Kuwait Oil Company (KOC) Hospital
P Ronnander et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 125, 54-63 (2018-09-18)
A mathematical model was developed to predict the transport of sumatriptan molecules across the skin followed by absorption into the bloodstream. The drug was encapsulated in dissolving polyvinylpyrrolidone-based microneedles shaped in the form of pyramids. Mass balance equations were derived
H Yamada et al.
The journal of obstetrics and gynaecology research, 24(2), 103-108 (1998-06-19)
A 24-year-old woman underwent conization and cervical cerclage during pregnancy. After she later complained of fluid leakage, a premature rupture of the membranes (PROM) was suspected, because of positive results on a test for nitrazine, the intra-amniotic dye injection method
Why certain dyes are useful for localizing the sentinel lymph node.
Tsopelas C and Sutton R
Journal of Nuclear Medicine, 43, 1377-1382 (2002)
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