199583
Lissamine™ Green B
Dye content 60 %
Synonym(s):
Acid Green 50, Wool Green S
About This Item
Recommended Products
form
powder
Quality Level
composition
Dye content, 60%
solubility
H2O: 0.1%
λmax
633 nm
ε (extinction coefficient)
≥10000 at 300-306 nm in water at 0.005 g/L
≥50000 at 236-242 nm in water at 0.005 g/L
≥60000 at 630-636 nm in water at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].CN(C)c1ccc(cc1)C(=C2/C=CC(\C=C2)=[N+](/C)C)\c3c(O)c(cc4cc(ccc34)S([O-])(=O)=O)S([O-])(=O)=O
InChI
1S/C27H26N2O7S2.Na/c1-28(2)20-9-5-17(6-10-20)25(18-7-11-21(12-8-18)29(3)4)26-23-14-13-22(37(31,32)33)15-19(23)16-24(27(26)30)38(34,35)36;/h5-16H,1-4H3,(H2,31,32,33,34,35,36);/q;+1/p-1
InChI key
WDPIZEKLJKBSOZ-UHFFFAOYSA-M
Related Categories
Application
Biochem/physiol Actions
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the ?max 199583, Lissamine™ Green B, in water?
The ?max in water is 633 nm.
What is the solubility of Product 199583, Lissamine™ Green B?
Lissamine Green B is soluble in water up to 0.1% in water. Other sources have stated solubility for some lots can approach 4%. Solubility in ethanol is 3 mg/mL, although this is not tested by Sigma Aldrich on a lot to lot basis. Lissamine Green B has also been reported to be soluble in Cellosolve, and in ethylene glycol.
What are possible uses for Product 199583, Lissamine™ Green B?
The Sigma Aldrich Handbook of Stains, Dyes and Indicators advises that Lissamine Green B has been substituted for Fast Green FCF without any real advantage. Lillie found it performed well as a collagen stain in Masson's trichrome and as a counter stain in the Van Gieson method. However, it was inferior when used as a component in Van Gieson's Trichrome Stain. See also Clark G, Staining Procedures, 4th Edition. (Williams & Wilkins, Baltimore, 1981), p. 120 for the Lillie modification of Masson's trichrome method. This material is used in the determination of chlorine dioxide in solution in EPA Method 32.
Has Product 199583, Lissamine™ Green B, been used for industrial applications?
The Sigma Aldrich Handbook of Stains, Dyes and Indicators states Lissamine Green B has been used to dye wool, silk (from a sulfuric acid bath), to print wool and silk directly and to coat paper, dye leather and surface stain wood.
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