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Merck
CN

198250

Rose bengal

certified by the BSC, Dye content ≥80 %

Synonym(s):

4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt

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About This Item

Empirical Formula (Hill Notation):
C20H2Cl4I4Na2O5
CAS Number:
632-69-9
Molecular Weight:
1017.64
NACRES:
NA.47
PubChem Substance ID:
24851870
UNSPSC Code:
12171500
Colour Index Number:
45440
EC Number:
211-183-3
MDL number:
MFCD00005043
Beilstein/REAXYS Number:
3645857

Key Documents

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Product Name

Rose bengal, certified by the BSC, Dye content ≥80 %

InChI key

UWBXIFCTIZXXLS-UHFFFAOYSA-L

InChI

1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

color

dark violet

solubility

H2O: 1 mg/mL

λmax

548 nm

ε (extinction coefficient)

≥90000 at 546-550 nm at 0.008 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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General description

Rose Bengal is the sodium salt of tetraiodotetrachlorouorescein, C20H2O5I4Cl4Na2. It is an anionic water-soluble xanthene dye.

Application

Rose bengal has several microbilogical staining applications including:
  • Conn′s technique for bacteria in soil and Kreyberg′s stain for keratin and mucus
  • counterstaining hematoxylin
  • staining cellular inclusions, together with Bismark brown



Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT0210
SHBS1232
0000253671
0000253671
SHBQ2115

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