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Safety Information

T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

Synonym(s):

TMB membrane substrate

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100 ML
CN¥763.86

CN¥763.86

List priceCN¥1,336.76Save 43%
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100 ML
CN¥763.86

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

CN¥763.86

List priceCN¥1,336.76Save 43%
Please contact Customer Service for Availability
Request a Bulk Order

Product Name

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, ready to use solution

Quality Level

100

form

liquid

storage temp.

2-8°C

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T0440T9455T8768
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes ready to use solution

T0565

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA peroxidase substrate

T0440

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA

TMB Enhanced One Component HRP Membrane Substrate

T9455

TMB Enhanced One Component HRP Membrane Substrate

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate powder, ≥98%

T8768

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

form

liquid

form

liquid

form

solution

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.[1]

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used as a chromogenic substrate in western blot analysis. It has also been used as a liquid substrate in enzyme-linked immunosorbent assay (ELISA).
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
  • enzyme-linked immune absorbent spot (ELISPOT) assay[2]
  • as a substrate for IgG peroxidase[3]
  • in the visualization of immunocomplexes[4]

Physical form

Ready-to-use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000426948
    0000426948
    0000391229
    0000391229
    0000358193

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    Hesham Saeed et al.
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    Microbial L-asparaginases are aminohydrolases that hydrolyze L-asparagine to L-aspartate. They are used to treat acute lymphoblastic leukemia and Hodgkin's lymphomas and in food industries. Increasing demand for L-ASNases is therefore needed. In the current study, the recombinant L-ASNase from Dickeya
    immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV
    Rascon-Castelo E, et al.
    Vaccines, 3(4), 973-987 (2015)
    Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma
    Steponaitis G, et al.
    Oncology Letters, 12(5), 3305-3311 (2016)
    Muhammed M Salahuddin et al.
    Current issues in molecular biology, 43(3), 2199-2209 (2021-12-24)
    Breast cancer is the most common malignancy in women worldwide. P2X7 is a transmembrane receptor expressed in breast cancer and activated by the ATP tumor microenvironment, driving cell proliferation, angiogenesis, and metastasis via different signaling pathways. The role of the
    Srdjan M Dragovic et al.
    Cell host & microbe, 23(4), 523-535 (2018-04-13)
    Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against
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    Colorimetric Phosphatase/Peroxidase Substrate Systems

    NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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