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SH1111

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock

Nuclear Hormone Receptors

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100 μL
CN¥5,551.31

CN¥5,551.31

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100 μL
CN¥5,551.31

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

CN¥5,551.31

Please contact Customer Service for Availability
Request a Bulk OrderRequest more information

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胆固醇 powder, BioReagent, suitable for cell culture, ≥99%

C3045

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胆固醇 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1533

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胆固醇 from sheep wool, ≥92.5% (GC), powder

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胆固醇 Sigma Grade, ≥99%

C8667

胆固醇

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

assay

≥99%

assay

-

assay

≥92.5% (GC)

assay

≥99%

biological source

ovine wool

biological source

-

biological source

sheep wool

biological source

sheep wool

solubility

H2O: 0.002 mg/mL, ethanol: soluble, Tween: soluble, serum: soluble

solubility

water: soluble 0.00003 g/L at 20 °C

solubility

water: soluble 0.00003 g/L at 20 °C

solubility

water: soluble 0.00003 g/L at 20 °C

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

-10 to -25°C

storage temp.

room temp

storage temp.

−20°C

General description

The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as 50 μl bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 15% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 218, Number of Clones: 1448
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Nuclear hormone receptor assays for drug discovery
    Rosen, J., et al.
    Current Opinion in Investigational Drugs, 6, 224-230 (2003)
    R C Ribeiro et al.
    Annual review of medicine, 46, 443-453 (1995-01-01)
    The nuclear hormone receptor gene superfamily encodes structurally related proteins that regulate transcription of target genes. These macromolecules include receptors for steroid and thyroid hormones, vitamins, and other proteins for which no ligands have been found. These receptors have modular
    Coactivators in assay design for nuclear hormone receptor drug discovery
    Chen, T., et al.
    Drug Delivery Technology, 1, 835-842 (2003)
    Miltiadis Kininis et al.
    Nuclear receptor signaling, 6, e005-e005 (2008-02-28)
    Recent genomic analyses of transcription factor binding, histone modification, and gene expression have provided a global view of transcriptional regulation by nuclear receptors (NRs) that complements an existing large body of literature on gene-specific studies. The picture emerging from these

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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