B6755
BIS-TRIS propane
≥99.0% (titration)
Synonym(s):
1,3-Bis[tris(hydroxymethyl)methylamino]propane, 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol)
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40 REACTIONS
CN¥2,618.03
160 REACTIONS
CN¥7,222.85
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40 REACTIONS
CN¥2,618.03
160 REACTIONS
CN¥7,222.85
About This Item
Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
Quality Level
Assay
≥99.0% (titration)
form
powder
storage condition
dry at room temperature
technique(s)
HPLC: suitable
color
white
useful pH range
6.3-9.5
pKa (25 °C)
(1) 6.8, (2) 9.0
mp
164-165 °C (lit.)
solubility
water: 30 % (w/w), clear, colorless
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Related Categories
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This Item | 11635379001 | LIG2 | DNALIGRO |
|---|---|---|---|
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche |
| parameter 37 °C optimum reaction temp. | parameter - | parameter - | parameter 4-22 °C optimum reaction temp. |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
General description
Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments.
Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).
Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).
Application
BIS-TRIS Propane may be used in the following studies:
- as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).[1]
- for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.[2]
- as a buffer during GeO2 mineralisation.[3]
- as a buffer component in a study pertaining to environmental research[4]
- as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes[5]
Features and Benefits
- Suitable for Biological and Biochemical Research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C)
- Highly soluble in water
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Y Satoh et al.
The Journal of biological chemistry, 256(20), 10272-10275 (1981-10-25)
In the presence of a salt in an excess amount, the oxidation of native MbO2 was enhanced considerably above the normal autoxidation in buffr alone with the formation of the corresponding MetMb . anion complex. This anion-induced oxidation of MbO2
Estefania Boix et al.
Dalton transactions (Cambridge, England : 2003), 43(44), 16902-16910 (2014-10-11)
We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based
Engineering the Organophosphorus Acid Anhydrolase Enzyme for Increased Catalytic Efficiency and Broadened Stereospecificity on Russian VX
Daczkowski CM et al.
Biochemistry, 54, 6423-6433 (2015)
S V Albers et al.
Journal of bacteriology, 181(14), 4285-4291 (1999-07-10)
The archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This transport activity involves a membrane-bound glucose-binding protein that interacts
Elisabeth Dohmann Chang et al.
Environmental science & technology, 55(10), 6848-6856 (2021-03-17)
Water pH is predicted to affect the uptake of ionizable pharmaceuticals in fish. The current study used an in vitro primary fish gill cell culture system to assess the effect of pH values in the range of 4.5-8.75 on the
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