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Key Documents

Safety Information

B6429

Sigma-Aldrich

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

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CN¥107,440.72

CN¥107,440.72

Estimated to ship onMay 12, 2025Details

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.54

CN¥107,440.72

Estimated to ship onMay 12, 2025Details

grade

Molecular Biology
for molecular biology

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

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PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID plasmid vector for molecular cloning

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PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID

PSF-OXB20-COOH-FLUC - C-TERMINAL FLUC TAG BACTERIAL PLASMID plasmid vector for molecular cloning

OGS3037

PSF-OXB20-COOH-FLUC - C-TERMINAL FLUC TAG BACTERIAL PLASMID

PSF-OXB20-FRCFP - CONSTITUTIVE BACTERIAL PROMOTER CFP PLASMID plasmid vector for molecular cloning

OGS601

PSF-OXB20-FRCFP - CONSTITUTIVE BACTERIAL PROMOTER CFP PLASMID

PSF-OXB20-DAGFP - BACTERIAL DAGFP PLASMID plasmid vector for molecular cloning

OGS245

PSF-OXB20-DAGFP - BACTERIAL DAGFP PLASMID

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

mol wt

size 5475 bp

mol wt

size 5501 bp

mol wt

size 4533 bp

mol wt

size 4533 bp

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.[1]
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents[1] to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting[2][3][4]
  • enzyme linked immunosorbent assay (ELISA)[5]
  • immunohistochemistry[6]

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000386787
0000377173
0000368122
0000377173
0000368122

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Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
M B Engelund et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(2), R300-R311 (2011-10-07)
Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
View All Related Papers

Protocols

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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