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11636120910
Roche
PCR ELISA, DIG-Labeling
sufficient for 50 reactions, kit of 1 (8 components), suitable for nucleic acid labeling
Synonym(s):
ELISA
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1 AMP
CN¥483.72
CN¥483.72
Estimated to ship onSeptember 15, 2025Details
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1 AMP
CN¥483.72
About This Item
UNSPSC Code:
41116133
CN¥483.72
Estimated to ship onSeptember 15, 2025Details
Recommended Products
form
solution
usage
sufficient for 50 reactions
packaging
kit of 1 (8 components)
manufacturer/tradename
Roche
technique(s)
nucleic acid labeling: suitable
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | P9599 | P8340 | P8215 |
|---|---|---|---|
| form DMSO solution | form DMSO solution | form DMSO solution | form DMSO solution |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
General description
The PCR ELISA, DIG-Labeling kit is for nonradioactive labeling of DNA with digoxigenin (DIG)-deoxyuridine triphosphate (dUTP) by polymerase chain reaction (PCR). We recommend the use of this kit in combination with the PCR ELISA, DIG-Detection kit. This kit is part of a complete PCR ELISA system. The PCR ELISA system allows for convenient detection of PCR products in a microplate format. The PCR ELISA allows the sensitive and specific detection of PCR products in solution. It is approximately 100 times more sensitive than conventional ethidium bromide-stained agarose gels.
Application
PCR ELISA, DIG-Labeling is used for the detection and quantification of the PCR product. The convenient enzyme linked immunosorbent assay (ELISA) format makes this kit particularly suitable for determining the presence or absence of a specific target sequence in a large number of samples. By combining with other reagents and equipment, this system can be used for many molecular biology applications. For example:
- In the presence of an appropriate target-specific, biotin-labeled capture probe, you can distinguish PCR products that differ by as little as a single base pair. Thus the PCR ELISA system (DIG-labeling + DIG-detection) can be used: to detect point mutations, deletions or insertions and to classify target sequences (e.g., as in HLA-typing or cell typing).
- Preparation of suitable DIG-labeled standards and addition of a colorimetric detection system will allow you to quantify PCR products.
- The microplate format used by the PCR ELISA system makes the system compatible with automated plate preparation and reading systems.
- The microplate format combined with colorimetric detection allows quantification of the amount of template present in a sample when used in combination with suitable standards.[1]
- When using appropriate capture probes, the kit can be used for the typing of a target sequence (e.g., in HLA-typing or cell typing). The microplate format makes this kit particularly suited for automation.
- The kit has been used in RT-PCR ELISA method to measure the dihydropyrimidine dehydrogenase (DPD) mRNA expression in liver and cell cycle phase distribution of bone marrow cells.[2]
Packaging
1 kit containing 8 components.
Quality
For best results run a negative control omitting template DNA to check for cross contamination from reagents.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- PCR DIG Labeling Mix, containing 2 mM dATP, dCTP, dGTP, 1.9 mM dTTP, and 0.1 mM Digoxigenin-dUTP
- PCR Reaction Buffer, without MgCl2, 100 mM Tris/HCl, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated
- MgCl2 Stock Solution 25 mM
- PCR Buffer, 100 mM Tris/HCl, 15 mM MgCl2, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated
- Taq DNA Polymerase, in storage buffer: 20 mM Tris/HCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.5% Nonidet P40 (v/v), 50% Tween 20 (v/v), 0.5% glycerol (v/v), pH 8.0 (+4 °C) 5 U/μl
- Control PCR Primer Mix, specific for the human t-PA gene 125 pmol each
- Human Control DNA 1 ng/μl
- Water, PCR Grade
See All (8)
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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A M Bailey et al.
FEMS microbiology letters, 207(2), 153-158 (2002-04-18)
The ribosomal internal transcribed spacer 1 region was sequenced for 10 species of Pythium and eight species of Phytophthora. Alignment of the sequences revealed considerable sequence microheterogeneity, which was utilized to prepare a capture probe of unique sequence for each
Elisabeth Filipski et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 287(4), R844-R851 (2004-06-26)
Rest-activity or cortisol rhythms can be altered in cancer patients, a condition that may impair the benefits from a timed delivery of anticancer treatments. In rodents, the circadian pattern in rest-activity is suppressed by the destruction of the suprachiasmatic nuclei
Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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