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11373242910

Roche

Fluorescein-12-dUTP

≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution

Synonym(s):

Fluorescein-12-dUTP, dUTP

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25 μL
CN¥5,079.29

CN¥5,079.29

Available to ship onApril 14, 2025Details

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25 μL
CN¥5,079.29

About This Item

UNSPSC Code:
41116100

CN¥5,079.29

Available to ship onApril 14, 2025Details

Quality Level

100

Assay

≥85% (HPLC)

form

solution

mol wt

Mr 1018.4 (fluorescein-12-dUTP-Li4)

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

technique(s)

ELISA: suitable
hybridization: suitable

storage temp.

−20°C

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This Item
809705A0410D4001
Fluorescein-12-dUTP ≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution

11373242910

Fluorescein-12-dUTP

5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

809705

5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

Aminoallyl-dUTP sodium salt ≥85%, lyophilized powder

A0410

Aminoallyl-dUTP sodium salt

2′-Deoxyuridine 5′-triphosphate sodium salt ≥95%

D4001

2′-Deoxyuridine 5′-triphosphate sodium salt

form

solution

form

solution

form

lyophilized powder

form

powder

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

assay

≥85% (HPLC)

assay

>95% (HPLC)

assay

≥85%

assay

≥95%

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

mol wt

Mr 1018.4 (fluorescein-12-dUTP-Li4)

mol wt

-

mol wt

-

mol wt

-

Application

Fluorescein-12-dUTP is used as a substrate for:
  • terminal transferase
  • DNA polymerase I (holoenzyme and Klenow fragment),
  • Taq DNA polymerase
  • reverse transcriptase (e.g., from AMV and M-MuLV).
Fluorescein-12-dUTP has been used to label nuclear extracts during PCR.[1] It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL.[2] Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection[3] or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Quality

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Physical form

Fluorescein-12-dUTP, tetralithium salt.

Storage and Stability

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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