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安全信息

M4526

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, sterile liquid, suitable for cell culture

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别名:
αMEM, MEM
UNSPSC代码:
12352207
NACRES:
NA.71

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

运输

ambient

储存温度

2-8°C

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一般描述

最小必需培养基Eagle（MEM）是一种合成细胞培养基，由Harry Eagle开发。它有更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸，可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM，含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸，其中一些的浓度有所增加。此外，它还含有5种额外的维生素。

应用

最小必需培养基Eagle已用于维持：
  • 小鼠未成熟的卵母细胞络合物
  • 人类间充质干细胞（MSC）
  • 小鼠成骨前细胞系

重悬

添加 0.292g/L L-谷氨酰胺。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
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Vision loss caused by photoreceptor death represents one of the first symptoms in neuronal ceroid lipofuscinosis, a condition characterized by accumulation of intracellular waste. Cln6nclf mice have a naturally occurring mutation in ceroid-lipofuscinosis neuronal (CLN) protein 6 and are a
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Current pulpotomy is limited in its ability to induce regeneration of the dental-pulp (DP) complex. Hydrogels are reported to be well-suited for tissue engineering and are unlikely to induce an inflammatory response that might damage the remaining tissue. The present
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Potency assays are critical for regenerative medicine, addressing the known challenge of functional heterogeneity among human multipotent stromal cells (hMSC). Necessary laboratory cell expansion allows analysis before implantation in the patient. Levels of induction of five signature gene biomarkers, ALPL
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