包装
pkg of 1 vial
运输
ambient
储存温度
−20°C
一般描述
The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA
应用
Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology
特点和优势
HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion.
外形
Dry
制备说明
Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
实验方案
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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