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安全信息

DUO92002

Sigma-Aldrich

Duolink® 原位PLA® 探针抗兔PLUS

Affinity purified Donkey anti-Rabbit IgG (H+L)

别名:

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.32

生物来源

donkey (polyclonal)

质量水平

200

抗体形式

affinity purified immunoglobulin (secondary antibody)

抗体产品类型

primary antibodies

产品线

Duolink®

种属反应性

rabbit

技术

immunofluorescence: suitable
proximity ligation assay: suitable

适用性

suitable for brightfield
suitable for fluorescence

运输

wet ice

储存温度

2-8°C

相关类别

应用

Duolink®邻位连接技术（PLA®） 能够在单分子水平下在固定细胞和组织样品中对蛋白质相互作用、翻译后修饰和蛋白质表达水平进行内源性检测。

该产品适用于 Duolink® 原位荧光检测方案 和 Duolink® 原位明场检测方案 ，具体取决于所用的检测试剂。

请访问我们的 Duolink® PLA资源中心 ，了解有关Duolink® 实验、应用、故障排除等方面的信息。

想要完成Duolink® PLA 原位 实验，您将需要两种一抗 （经过PLA、IHC、ICC或IF验证） 以识别两个目标表位。其他必需试剂包括来自不同物种的一对PLA探针（一个PLUS和一个MINUS）、检测试剂、洗涤缓冲液和封固剂。请注意，一抗必须来自与Duolink® PLA探针相同的物种。使用标准免疫荧光测定设备进行分析。HRP也可用于明场检测。

特异性
PLA探针抗兔抗体与全分子兔IgG以及其他兔免疫球蛋白的轻链反应。PLA探针抗兔抗体与牛、鸡、山羊、豚鼠、叙利亚仓鼠、马、人、兔和绵羊血清蛋白质具有极小的交叉反应性。 与本产品同时使用的MINUS探针必须来自不同物种。有关更多信息，参见我们的 产品选择指南 。

应用说明
需要使用来自不同物种的两种一抗。在标准免疫荧光（IF）、免疫组织化学（IHC）或免疫细胞化学（ICC）测定中测试一抗（IgG类，单克隆或多克隆），以确定最佳的固定、封闭和滴度条件。Duolink® PLA 原位 试剂适用于固定细胞、细胞离心涂片细胞、载玻片上生长的细胞、福尔马林固定石蜡包埋（FFPE）样本或组织（新鲜或冷冻）。无最少细胞数量限制。

让我们帮您完成工作，了解有关 定制服务计划 的更多信息，加速您的Duolink® 项目

查看完整的Duolink® 产品列表
特异性
抗兔PLA探针可与全分子兔IgG和其它兔免疫球蛋白的轻链反应。PLA探针抗兔抗体与牛、鸡、山羊、豚鼠、叙利亚仓鼠、马、人、兔和绵羊血清蛋白质具有极小的交叉反应性。本产品须配合不同物种的MINUS探针使用。请参见我们的 产品选择指南 以获得更多信息。

应用说明
需要使用在不同物种中产生的两种一抗。在标准免疫荧光（IF）、免疫组织化学（IHC）或免疫细胞化学（ICC）检测中测试您的一抗（IgG类，单克隆或多克隆），以确定最佳的固定、封闭和滴度条件。Duolink® PLA 原位试剂适用于固定细胞、细胞离心涂片、载玻片上生长的细胞、福尔马林固定石蜡包埋 (FFPE) 或组织（新鲜或冷冻）。无最小细胞数量要求。

让我们为您完成相关工作，了解更多关于我们定制服务计划的相关信息，以加速您的Duolink®项目

查看完整的Duolink®产品列表

特点和优势

  • 不需要过表达或基因操纵
  • 特异性高（假阳性较少）
  • 因滚环扩增而具有单分子检测能力
  • 相对量化可能性
  • 无需特殊设备
  • 比FRET更快、更简单
  • 准确性高于co-IP
  • 所得结果可立即发表

组分

该产品包含以下组分：
  • 5xPLA探针抗兔PLUS - 寡核苷酸PLUS偶联的驴抗兔二抗
  • 1x封闭液 - 用于阻断样品的试剂
  • 1x抗体稀释液 - 用于稀释PLA探针和一抗
有关更多信息，参见产品数据表。

其他说明

2024年CiteAb奖，授予在帕金森研究方面取得成功的供应商

法律信息

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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Try our 产品选型工具.

象形图

Exclamation markEnvironment
GHS07,GHS09

警示用语：

Warning

危险声明

H317,H411

危险分类

Aquatic Chronic 2 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What epitope on the antibody is recognized by the Duolink PLA Probes?

    The Duolink PLA Probes offered are donkey polyclonal antibodies directed to the whole molecule (heavy and light chains).  They are affinity purified.  The same polyclonal antibody is conjugated to the Plus and Minus Oligos. The Plus and Minus probes can be used together (to detect a single antibody) since they can recognize multiple sites.  The anti-mouse IgG PLA labeled antibodies may cross-react with rat but this is not consistent. Do not use this product with rat antibodies.  It is best to label anti-rat secondary antibodies with the Duolink Probemaker kit.The anti-goat IgG PLA labeled antibodies have cross-reactivity with sheep IgG and can be recommended for this application.

  6. Has Duolink been used for detection of changes in protein expression?

    The following reference cites the use of Duolink to detect changes in protein expression in Human. Raizer et al., Cancer 2010;116:5297-305

  7. How many reactions can be performed with the Duolink® In Situ Starter Kit?

    All of the starter kits for the Duolink product line will perform 30 reactions using 40 ul per reaction.    DUO92101 - Duolink® In Situ Red Starter Kit Mouse/Rabbit    DUO92102 - Duolink® In Situ Orange Starter Kit Mouse/Rabbit    DUO92103 - Duolink® In Situ Red Starter Kit Mouse/Goat    DUO92104 - Duolink® In Situ Orange Starter Kit Mouse/Goat    DUO92105 - Duolink® In Situ Red Starter Kit Goat/Rabbit    DUO92106 - Duolink® In Situ Orange Starter Kit Goat/Rabbit

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Xiaofan Li et al.
PLoS pathogens, 13(3), e1006249-e1006249 (2017-03-02)
Trials to reintroduce chloroquine into regions of Africa where P. falciparum has regained susceptibility to chloroquine are underway. However, there are long-standing concerns about whether chloroquine increases lytic-replication of Epstein-Barr virus (EBV), thereby contributing to the development of endemic Burkitt
M Aubele et al.
British journal of cancer, 103(5), 663-667 (2010-08-12)
Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression
Ivan Matic et al.
Molecular cell, 39(4), 641-652 (2010-08-28)
Reversible protein modification by small ubiquitin-like modifiers (SUMOs) is critical for eukaryotic life. Mass spectrometry-based proteomics has proven effective at identifying hundreds of potential SUMO target proteins. However, direct identification of SUMO acceptor lysines in complex samples by mass spectrometry
Pengcheng Zhu et al.
Cancer cell, 19(3), 401-415 (2011-03-15)
Cancer is a leading cause of death worldwide. Tumor cells exploit various signaling pathways to promote their growth and metastasis. To our knowledge, the role of angiopoietin-like 4 protein (ANGPTL4) in cancer remains undefined. Here, we found that elevated ANGPTL4
John J Peluso et al.
Endocrinology, 153(8), 3929-3939 (2012-06-22)
Progesterone (P4) receptor membrane component (PGRMC)1 is detected as a 22-kDa band as well as higher molecular mass bands (>50 kDa) in spontaneously immortalized granulosa cells. That these higher molecular mass bands represent PGRMC1 is supported by the findings that
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