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Merck
CN
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文件

安全信息

DIF001

Sigma-Aldrich

3T3-L1分化试剂盒

Sufficient for 100 mL differentiation medium

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UNSPSC代码:
12352207
NACRES:
NA.75

形式

solution

用途

100 mL differentiation medium

技术

cell culture | mammalian: suitable

运输

wet ice

储存温度

−20°C

一般描述

3T3-L1细胞衍生自小鼠3T3细胞，可在脂肪生理学和代谢疾病研究中提供一种被广泛使用的基础模型。它们在分化之前表现出成纤维细胞样形态，但在分化开始几天后则变成圆形并积聚脂滴。累积的脂滴可通过光学显微镜而观察到。体外分化的3T3-L1脂肪细胞可产生与组织来源的脂肪细胞类似的特征，并通常用于研究脂肪生成、脂肪分解和代谢功能障碍。3T3-L1分化试剂盒可提供足够的补充剂以制备100 ml分化培养基和600 ml维持培养基，从而足够用于12个100 mm培养皿。

应用

3T3-L1分化试剂盒已用于：
  • 3T3-L1前脂肪细胞向脂肪细胞的分化。
  • 研究肥胖、脂肪生成、脂肪分解和脂质代谢
  • 3T3-L1前脂肪细胞向脂肪细胞的分化
  • 研究肥胖、脂肪生成、脂肪分解和脂质代谢。

组分

胰岛素 (1.5 mg/ml) 0.6 ml (货号DIF001A，绿盖)
分化混合物，1000x (冻干的) 1 vl (货号DIF001B，黄盖)
DMSO (无水) 0.5 ml (货号DIF001C，蓝盖)

储存及稳定性

将试剂盒在−20 °C避光保存。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H360FD

危险分类

Repr. 1B

WGK

WGK 3

法规信息

监管及禁止进口产品

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