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solution
用途
100 mL differentiation medium
技术
cell culture | mammalian: suitable
运输
wet ice
储存温度
−20°C
一般描述
3T3-L1细胞衍生自小鼠3T3细胞,可在脂肪生理学和代谢疾病研究中提供一种被广泛使用的基础模型。它们在分化之前表现出成纤维细胞样形态,但在分化开始几天后则变成圆形并积聚脂滴。累积的脂滴可通过光学显微镜而观察到。体外分化的3T3-L1脂肪细胞可产生与组织来源的脂肪细胞类似的特征,并通常用于研究脂肪生成、脂肪分解和代谢功能障碍。3T3-L1分化试剂盒可提供足够的补充剂以制备100 ml分化培养基和600 ml维持培养基,从而足够用于12个100 mm培养皿。
应用
3T3-L1分化试剂盒已用于:
- 3T3-L1前脂肪细胞向脂肪细胞的分化。
- 研究肥胖、脂肪生成、脂肪分解和脂质代谢
- 3T3-L1前脂肪细胞向脂肪细胞的分化
- 研究肥胖、脂肪生成、脂肪分解和脂质代谢。
组分
胰岛素 (1.5 mg/ml) 0.6 ml (货号DIF001A,绿盖)
分化混合物,1000x (冻干的) 1 vl (货号DIF001B,黄盖)
DMSO (无水) 0.5 ml (货号DIF001C,蓝盖)
分化混合物,1000x (冻干的) 1 vl (货号DIF001B,黄盖)
DMSO (无水) 0.5 ml (货号DIF001C,蓝盖)
储存及稳定性
将试剂盒在−20 °C避光保存。
警示用语:
Danger
危险声明
危险分类
Repr. 1B
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
监管及禁止进口产品
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