应用
二喹啉甲酸试剂盒被用来估量唾液蛋白组样本,人胚肾293细胞和黄体细胞中的蛋白质。
特点和优势
- 简单、灵敏的比色法蛋白质检测
- 两种稳定试剂作为工作溶液
- 相对于Lowry蛋白检测,速度更快,操作更轻松
- 兼容多种离子型及非离子型去垢剂
- 相对于Bradford染料结合检测,不同蛋白质间的差异更小
- 可针对微孔板应用进行灵活调整
原理
蛋白质将碱性Cu(II)还原为Cu(I)具有浓度依赖性。二辛可宁酸是一种应用于Cu(I)的高度特异性显色试剂,可以形成在562 nm处具有最高吸光度的紫色复合物。吸光度与蛋白浓度成正比。这是用于蛋白测定的Folin-Ciocalteu试剂的替代方法。
仅试剂盒组分
产品编号
说明
- Bicinchoninic Acid Solution 1 L
- 4%(w/v) CuSO4 · 5H2O Solution 25 mL
- Protein Standard Solution 5 x 1
应用
产品编号
说明
价格
警示用语:
Danger
危险声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
含少量动物源组分生物产品
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相关内容
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