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Merck
CN

BCA1

Sigma-Aldrich

BCA(Bicinchoninic Acid)蛋白定量试剂盒

for 200-1000 μg/ml protein

别名:

BCA蛋白检测

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About This Item

UNSPSC代码:
12352106
NACRES:
NA.32

质量水平

用途

sufficient for ~500 assays

储存温度

2-8°C

应用

二喹啉甲酸试剂盒被用来估量唾液蛋白组样本,人胚肾293细胞和黄体细胞中的蛋白质。

特点和优势

  • 简单、灵敏的比色法蛋白质检测
  • 两种稳定试剂作为工作溶液
  • 相对于Lowry蛋白检测,速度更快,操作更轻松
  • 兼容多种离子型及非离子型去垢剂
  • 相对于Bradford染料结合检测,不同蛋白质间的差异更小
  • 可针对微孔板应用进行灵活调整

原理

蛋白质将碱性Cu(II)还原为Cu(I)具有浓度依赖性。二辛可宁酸是一种应用于Cu(I)的高度特异性显色试剂,可以形成在562 nm处具有最高吸光度的紫色复合物。吸光度与蛋白浓度成正比。这是用于蛋白测定的Folin-Ciocalteu试剂的替代方法。

仅试剂盒组分

产品编号
说明

  • Bicinchoninic Acid Solution 1 L

  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL

  • Protein Standard Solution 5 x 1

应用

产品编号
说明
价格

象形图

CorrosionEnvironment

警示用语:

Danger

危险声明

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

含少量动物源组分生物产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Tianeptine (Tian) possesses neuroprotective potential, however, little is known about the effect of this drug in models of neuronal apoptosis. In the present study, we aimed (1) to compare the neuroprotective capacities of some antidepressants (ADs) in the models of
TGFB1 modulates in vitro secretory activity and viability of equine luteal cells
Galvao A, et al.
Cytokine, 110, 316-327 (2018)
Salivary proteome patterns of individuals exposed to High Altitude
Jain S, et al.
Archives of Oral Biology, 96, 104-112 (2018)
Human OATP1B1 (SLCO1B1) transports sulfated bile acids and bile salts with particular efficiency
Toth B, et al.
Toxicology in vitro, 52, 189-194 (2018)
Emily K Davenport et al.
Antimicrobial agents and chemotherapy, 58(8), 4755-4761 (2014-06-11)
We investigated biofilms of two pathogens, Acinetobacter baumannii and Staphylococcus aureus, to characterize mechanisms by which the extracellular polymeric substance (EPS) found in biofilms can protect bacteria against tobramycin exposure. To do so, it is critical to study EPS-antibiotic interactions

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Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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