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Merck
CN

B6404

Supelco

BCIP®/NBT 溶液,预混合

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UNSPSC代码:
41105333
NACRES:
NA.32

应用

BCIP/NBT 溶液,经过预混合后:
  • 作为底物用于斑点印迹显影
  • 作为底物用于在免疫组织化学分析中呈现碱性磷酸酶
  • 在蛋白质印迹分析中检测特异性免疫复合物

其他说明

0.48mM NBT,0.56mM BCIP,10mM Tris 和 59.3mM MgCl2,pH 约 9.2

底物

用于在蛋白质印迹中显现碱性磷酸酶缀合物的底物。

法律信息

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Slow-and fast-twitch rat hind limb skeletal muscle phenotypes 8 months after spinal cord transection and olfactory ensheathing glia transplantation
Negredo P, et al.
The Journal of Physiology, 586(10), 2593-2610 (2008)
Dietary iron concentration may influence aging process by altering oxidative stress in tissues of adult rats
Arruda L F, et al.
PLoS ONE, 8(4), e61058-e61058 (2013)

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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