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形式
liquid
质量水平
应用
BCIP/NBT 溶液,经过预混合后:
- 作为底物用于斑点印迹显影
- 作为底物用于在免疫组织化学分析中呈现碱性磷酸酶
- 在蛋白质印迹分析中检测特异性免疫复合物
其他说明
0.48mM NBT,0.56mM BCIP,10mM Tris 和 59.3mM MgCl2,pH 约 9.2
底物
用于在蛋白质印迹中显现碱性磷酸酶缀合物的底物。
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Journal of Molecular Biology, 343(3), 759-769 (2004)
Slow-and fast-twitch rat hind limb skeletal muscle phenotypes 8 months after spinal cord transection and olfactory ensheathing glia transplantation
The Journal of Physiology, 586(10), 2593-2610 (2008)
Dietary iron concentration may influence aging process by altering oxidative stress in tissues of adult rats
PLoS ONE, 8(4), e61058-e61058 (2013)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
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