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P8203

Sigma-Aldrich

哌嗪-N,N'-二(2-乙磺酸)

BioXtra, ≥99% (titration)

别名:

1,4-哌嗪二乙磺酸, 哌嗪-1,4-双(2-乙磺酸), 哌嗪-N,N′-双(2-乙磺酸)

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¥3,231.30
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¥10,383.63

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50 G
¥3,231.30
250 G
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About This Item

经验公式（希尔记法）:
C8H18N2O6S2
CAS号:
5625-37-6
分子量:
302.37
Beilstein:
817713
EC 号:
227-057-6
MDL编号:
MFCD00006159
UNSPSC代码:
12161700
PubChem化学物质编号:
24898917
NACRES:
NA.25

¥3,231.30

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产品线

BioXtra

方案

≥99% (titration)

表单

powder

杂质

Insoluble matter, passes filter test

灼烧残渣 (900 °C)

≤0.5% (as SO4)

缺失

≤0.5% loss on drying, 110°C

有效pH范围

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

溶解性

1 M NaOH: 0.5 M at 20 °C, clear, colorless

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此商品
P6757P185180635
哌嗪-N,N'-二(2-乙磺酸) BioXtra, ≥99% (titration)

P8203

哌嗪-N,N'-二(2-乙磺酸)

哌嗪-N,N'-二(2-乙磺酸) ≥99% (titration)

P6757

哌嗪-N,N'-二(2-乙磺酸)

哌嗪-N,N′-二(2-乙磺酸) BioPerformance Certified, suitable for cell culture

P1851

哌嗪-N,N′-二(2-乙磺酸)

哌嗪-N,N′-二(2-乙磺酸) BioXtra, ≥99.5% (T)

80635

哌嗪-N,N′-二(2-乙磺酸)

product line

BioXtra

product line

-

product line

-

product line

BioXtra

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

form

powder

form

crystalline powder

form

crystalline powder

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

一般描述

PIPES是乙磺酸缓冲盐系列之一，由Good等人首先开发[1] 以满足某些标准：中值pKa，最大水溶性和在所有其他溶剂中的最小溶解度，最小盐效应，pKa随温度的变化最小，化学和酶学稳定，在可见光或紫外光谱范围的最小吸收，易于合成。由于它在37 °C的pKa接近生理pH，PIPES还可用于细胞培养工作。

应用

使用PIPES用于分离琼脂糖凝胶中的乙醛酸化RNA、核酸酶S1的RNA作图以及核糖核酸酶保护检测的实验方法已被报道。[2]PIPES已被用作戊二醛固定组织样品的缓冲液。[3],[4]
PIPES已被用于蛋白结晶。[5]，[6] PIPES在tubulin
α和β亚基在免疫吸附凝胶上解析后进行重构的应用已被描述。[7]PIPES已被推荐用于半胱天冬酶3、6、7和8体外研究的缓冲液中。[8]
一项已发表的研究表明了PIPES在蛋白质分析等应用中可作为非金属离子络合缓冲液。[9]PIPES在细胞培养中已被用于如在大肠杆菌中对热稳定突变膜蛋白进行工程化改造等应用。[10]

质量

已在BioXtra PIPES上进行了痕量元素分析；分析证书提供了特定批次的结果。BioXtra PIPES适用于需要严格控制元素含量的应用。

联系

Sigma-Aldrich公司提供生物性能认证的细胞培养用PIPES(产品编号 P1851) 以及其他几种不同的盐类，以为缓冲液制备提供便利。

制备说明

缓冲液制备可通过向PIPES游离酸中加入碱溶液，滴定到适当的pH值，或采用等摩尔的钠盐溶液和二钠盐溶液混合滴定到适当的pH值。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000425944
    0000425944
    0000426344
    0000426344
    0000407767

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    如果您需要特殊版本，可通过批号或批次号查找具体证书。

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    S Haviernick et al.
    Journal of microscopy, 135(Pt 1), 83-88 (1984-07-01)
    It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy
    S A Moore et al.
    Journal of molecular biology, 312(3), 511-523 (2001-09-21)
    Human bile salt-stimulated lipase (BSSL), which is secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk, is important for the effective absorption of dietary lipids. The dependence of BSSL on bile acids
    Sambrook , J. and Russell, D.W.
    Molecular Cloning: A Laboratory Manual, 7-7 (2001)
    K E Loesser et al.
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(11), 1477-1485 (1986-11-01)
    We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia
    A Giraudel et al.
    Biochemistry, 37(24), 8724-8734 (1998-06-24)
    The dissociation and separation of the tubulin alpha- and beta-subunits have been achieved by binding alpha-subunits to an immunoadsorbent gel and selectively inducing release of free beta-subunits. The immunoadsorbent gel was prepared by coupling the monoclonal antibody YL1/2 to Sepharose
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    实验方案

    细胞免疫荧光标记

    细胞染色可分为四个步骤：细胞制备、固定、抗体孵育和评估。

    Immunofluorescence Labeling of Cells

    Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

    Restriction Enzyme Cloning Manual Buffer Recipes

    TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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