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Sigma-Aldrich

酶蛋白去糖基化试剂盒

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别名:
Glycosylation Release Kit
UNSPSC代码:
12352200
NACRES:
NA.32

偶联物

(N-linked)
 (O-linked)

质量水平

200

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

酶蛋白去糖基化试剂盒包含从糖蛋白中完整去除N连接和单一O连接碳水化合物所需的所有酶和试剂。该试剂盒用于裂解Core 2 O连接碳水化合物复合物，包括含有聚乳糖胺的碳水化合物。

应用

酶促蛋白去糖基化试剂盒用于：
  • 蛋白质去糖基化
  • 组织因子途径抑制因子(TFPI)去糖基化α以及β
  • 天然exo-β-(1,3)-葡聚糖酶EXG1的去糖基化

酶解蛋白去糖基化试剂盒已用于各种酶的去糖基化。

特点和优势

酶蛋白去糖基化试剂盒具有以下特点：
  • 适用于两mg以上规模的糖蛋白去糖基化
  • 中性pH值下单步反应
  • 天然&变性过程
  • 蛋白质无降解
  • 去除含聚唾液酸的O连接糖
  • 随附对照糖蛋白

仅试剂盒组分

产品编号
说明
  • PNGase F 1 mL/vial
  • O-Glycosidase 20 μL
  • α-2(3,6,8,9)-Neuraminidase 20 μL
  • Fetuin Control .5 mg
  • 5× Reaction Buffer .2 mL
  • Denaturation Solution .1 mL
  • Triton™ X-100 .1 mL
  • β-1→4-Galactosidase, positionally specific 20 μL
  • β-N-Acetylglucosaminidase 20 μL
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价格

警示用语：

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Noémie Caillot et al.
The Journal of allergy and clinical immunology, 140(3), 759-770 (2016-12-15)
Eligibility to immunotherapy is based on the determination of IgE reactivity to a specific allergen by means of skin prick or in vitro testing. Biomarkers predicting the likelihood of clinical improvement during immunotherapy would significantly improve patient selection. Proteins were differentially
Julien Chapuis et al.
Human molecular genetics, 20(10), 2026-2036 (2011-03-02)
Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by cerebral deposition of amyloid-β (Aβ), a series of peptides derived from the processing of the amyloid-β precursor protein (APP). To identify new candidate genes for AD, we recently performed
Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families
David K Crockett et al.
International journal of peptides, 2012, 621329-621329 (2012-04-27)
Histoplasmosis is a severe dimorphic fungus infection, which is often difficult to diagnose due to similarity in symptoms to other diseases and lack of specific diagnostic tests. Urine samples from histoplasma-antigen-positive patients and appropriate controls were prepared using various sample
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