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Merck
CN

EDEGLY

Sigma-Aldrich

酶蛋白去糖基化试剂盒

别名:

Glycosylation Release Kit

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

偶联物

(N-linked)
(O-linked)

质量水平

运输

wet ice

储存温度

2-8°C

一般描述

酶蛋白去糖基化试剂盒包含从糖蛋白中完整去除N连接和单一O连接碳水化合物所需的所有酶和试剂。该试剂盒用于裂解Core 2 O连接碳水化合物复合物,包括含有聚乳糖胺的碳水化合物。

应用

酶促蛋白去糖基化试剂盒用于:
  • 蛋白质去糖基化
  • 组织因子途径抑制因子(TFPI)去糖基化α以及β
  • 天然exo-β-(1,3)-葡聚糖酶EXG1的去糖基化

酶解蛋白去糖基化试剂盒已用于各种酶的去糖基化。

特点和优势

酶蛋白去糖基化试剂盒具有以下特点:
  • 适用于两mg以上规模的糖蛋白去糖基化
  • 中性pH值下单步反应
  • 天然&变性过程
  • 蛋白质无降解
  • 去除含聚唾液酸的O连接糖
  • 随附对照糖蛋白

仅试剂盒组分

产品编号
说明

  • PNGase F 1 mL/vial

  • O-Glycosidase 20 μL

  • α-2(3,6,8,9)-Neuraminidase 20 μL

  • Fetuin Control .5 mg

  • 5× Reaction Buffer .2 mL

  • Denaturation Solution .1 mL

  • Triton X-100 .1 mL

  • β-1→4-Galactosidase, positionally specific 20 μL

  • β-N-Acetylglucosaminidase 20 μL

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相关产品

产品编号
说明
价格

警示用语:

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Scabies mite peritrophins are potential targets of human host innate immunity
Mika A, et al.
PLoS Neglected Tropical Diseases, 5(9), e1331-e1331 (2011)
Primavera Alvarado et al.
Journal of microbiological methods, 175, 105981-105981 (2020-06-15)
The diagnosis of histoplasmosis depends on various approaches: direct clinical examination, fungus isolation from cultures of clinical samples, histopathological evaluation, and serological testing. In serodiagnostic assays, the Histoplasma capsulatum H and M antigenic glycoproteins have been extensively used. However, both
Guillermo Bernabó et al.
PloS one, 8(7), e71192-e71192 (2013-08-08)
Among the several multigene families codified by the genome of T. cruzi, the TcTASV family was the latest discovered. The TcTASV (Trypomastigote, Alanine, Serine, Valine) family is composed of ∼40 members, with conserved carboxi- and amino-termini but with a variable
Axel T Lehrer et al.
Vaccine, 36(22), 3090-3100 (2017-02-22)
Infections with filoviruses in humans are highly virulent, causing hemorrhagic fevers which result in up to 90% mortality. In addition to natural infections, the ability to use these viruses as bioterrorist weapons is of significant concern. Currently, there are no
Identification of histoplasma-specific peptides in human urine
Crockett DK, et al.
International Journal of Peptides, 2012 (2012)

商品

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

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