product name
星形孢菌素链霉菌sp., Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).
质量水平
检测方案
≥99% (HPLC)
形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
protect from light
颜色
white to pale yellow
溶解性
DMSO: 5 mg/mL
运输
ambient
储存温度
2-8°C
InChI
1S/C28H26N4O3/c1-28-26(34-3)17(29-2)12-20(35-28)31-18-10-6-4-8-14(18)22-23-16(13-30-27(23)33)21-15-9-5-7-11-19(15)32(28)25(21)24(22)31/h4-11,17,20,26,29H,12-13H2,1-3H3,(H,30,33)/t17-,20-,26-,28+/m1/s1
InChI key
HKSZLNNOFSGOKW-FYTWVXJKSA-N
一般描述
一种有效的、细胞可渗透性、可逆性、ATP竞争性广谱蛋白激酶抑制剂。可抑制CaM激酶（IC50 = 20 nM）、肌球蛋白轻链激酶（IC50 = 1.3 nM）、蛋白激酶A（IC50 = 7 nM）、蛋白激酶C（IC50 = 0.7 nM）和蛋白激酶G（IC50 = 8.5 nM）。还可抑制胶原蛋白或ADP诱导的血小板凝集，但对凝血酶诱导的血小板凝集没有影响。诱导人恶性神经胶质瘤细胞系凋亡。在G1检查点对正常细胞进行阻滞。
一种有效的、细胞可渗透性、可逆性、ATP竞争性广谱蛋白激酶抑制剂。可抑制蛋白激酶A（IC50 = 7 nM）、CaM激酶（IC50 = 20 nM）、肌球蛋白轻链激酶（IC50 = 1.3 nM）、蛋白激酶C（IC50 = 700 pM）和蛋白激酶G（IC50 = 8.5 nM）。还可抑制胶原蛋白或ADP诱导的血小板凝集，但对凝血酶诱导的血小板凝集没有影响。诱导人恶性神经胶质瘤细胞系凋亡。在G1检查点对正常细胞进行阻滞。可提供1 mM 星形孢菌素（100 µg/214µl）（货号569396）的DMSO溶液。
生化/生理作用
产物与ATP竞争。
可逆性：是
细胞可渗透性：具有
靶标IC50：针对蛋白激酶A、CaM激酶、肌球蛋白轻链激酶、蛋白激酶C和蛋白激酶G分别为7 nM、20 nM、1.3 nM、700 pM和8.5 nM。
首要靶标
PKA
PKA
包装
用惰性气体包装
警告
毒性：标准处理（A）
制备说明
仅在使用前再用水相缓冲液进行稀释。
重悬
在重悬后分装并冻存于冰箱（-70°C）。储备液可在-70°C最长稳定6个月。
其他说明
Couldwell, W.T., et al. 1994.FEBS Lett.345, 43.
Nishimura, H. and Simpson, I.A.1994.生物化学。J. 302, 271.
Bruno, S., et al. 1992.Cancer Res.52, 470.
Kiss, Z. and Deli, E. 1992.生物化学。J. 288, 853.
Vitale, M.L., et al. 1992.Neuroscience51, 463.
Hoffman, R. and Newland, E.S.1991.Cancer Chemother.Pharmacol.28, 102.
Oka, S., et al. 1986.Agric. Biol. Chem.50, 2723.
Nishimura, H. and Simpson, I.A.1994.生物化学。J. 302, 271.
Bruno, S., et al. 1992.Cancer Res.52, 470.
Kiss, Z. and Deli, E. 1992.生物化学。J. 288, 853.
Vitale, M.L., et al. 1992.Neuroscience51, 463.
Hoffman, R. and Newland, E.S.1991.Cancer Chemother.Pharmacol.28, 102.
Oka, S., et al. 1986.Agric. Biol. Chem.50, 2723.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 4 - Repr. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Laura Martínez-Escardó et al.
Cancers, 13(21) (2021-11-14)
Glioblastoma (GBM) is a highly aggressive brain tumor and almost all patients die because of relapses. GBM-derived cells undergo cell death without nuclear fragmentation upon treatment with different apoptotic agents. Nuclear dismantling determines the point-of-no-return in the apoptotic process. DFF40/CAD
Wenjia Xie et al.
Cell death & disease, 14(10), 663-663 (2023-10-11)
An important pathophysiological process of acute kidney injury (AKI) is mitochondrial fragmentation in renal tubular epithelial cells, which leads to cell death. Pyruvate kinase M2 (PKM2) is an active protein with various biological functions that participates in regulating glycolysis and
Alain Pulfer et al.
eLife, 12 (2024-03-18)
Intravital microscopy has revolutionized live-cell imaging by allowing the study of spatial-temporal cell dynamics in living animals. However, the complexity of the data generated by this technology has limited the development of effective computational tools to identify and quantify cell
Aneese A Jaffa et al.
Frontiers in pharmacology, 12, 743059-743059 (2021-12-07)
Microglia, the resident phagocytes of the central nervous system and one of the key modulators of the innate immune system, have been shown to play a major role in brain insults. Upon activation in response to neuroinflammation, microglia promote the
Xiaotong Lou et al.
Oxidative medicine and cellular longevity, 2021, 9397960-9397960 (2021-09-24)
In glaucomatous eyes, the main aqueous humor (AH) outflow pathway is damaged by accumulated oxidative stress arising from the microenvironment, vascular dysregulation, and aging, which results in increased outflow resistance and ocular hypertension. Schlemm's canal (SC) serves as the final
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门