质量水平
ligand
diethylaminoethyl
描述
weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
无菌性
sterile (Caustic Stable)
产品线
Fractogel®
形式
resin
参数
170 cm/hr flow rate
8 bar max. pressure
基质活性基团
methacrylate
平均粒径
40-90 μm
容量
100 mg binding capacity (BSA/mg of resin)
转变温度
flash point 35 °C (calculated)
密度
1.430 g/cm3
堆积密度
1000 kg/m3
应用
gene therapy
recombinant protein
vaccine development
分离技术
weak anion exchange
储存温度
2-30°C
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一般描述
特点和优势
- Excellent binding to large viruses and plasmid DNA
- Homogenous binding with high selectivity and purity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
包装
- 1.16884.0100: Fractogel® EMD DMAE (M) Resin 100ml
- 1.16884.0010: Fractogel® EMD DMAE (M) Resin 10ml
- 1.16884.0500: Fractogel® EMD DMAE (M) Resin 500ml
- 1.16884.5000: Fractogel® EMD DMAE (M) Resin 5L
分析说明
Microscopic evaluation: Uniform spherical particles,no agglomerates, no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin
法律信息
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警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
95.0 °F
闪点(°C)
35 °C
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