V900497
胃蛋白酶 来源于猪胃粘膜
Vetec™, reagent grade
别名:
胃液素 来源于猪胃, 胃蛋白酶 A
登录 查看组织和合同定价。
选择尺寸
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-629-3
MDL number:
Specific activity:
400-800 units/mg protein
grade
reagent grade
product line
Vetec™
form
solid
specific activity
400-800 units/mg protein
mol wt
35 kDa
正在寻找类似产品? 访问 产品对比指南
Application
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
Biochem/physiol Actions
优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键
Analysis Note
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
Other Notes
用血红蛋白作为底物，对TCA可溶性产物进行测定，在pH2.0，37℃条件下，一个单位每分钟可产生0.001 A280。
Legal Information
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Liu Lin et al.
Frontiers in nutrition, 9, 833712-833712 (2022-03-01)
The effects of condiment (salt) and processing technic (homogenization) on digestion and interfacial properties of micro/nano-sized colloidal particles (MNCPs) in bigeye tuna head soup (BTHS) using simulated gastrointestinal digestion model in vitro were investigated. For MNCPs in BTHS, the triglycerides
María Llarena-Reino et al.
Veterinary parasitology, 191(3-4), 276-283 (2012-10-10)
During the last 50 years human anisakiasis has been rising while parasites have increased their prevalence at determined fisheries becoming an emergent major public health problem. Although artificial enzymatic digestion procedure by CODEX (STAN 244-2004: standard for salted Atlantic herring
M N James et al.
Nature, 319(6048), 33-38 (1986-01-02)
The only well-understood mechanism of zymogen activation is that of the serine proteinases, in which proteolytic cleavage leads to conformational changes resulting in a functional active site. A different mechanism is now unveiled by the crystal structure of pepsinogen. Salt
Martial Rey et al.
Molecular & cellular proteomics : MCP, 12(2), 464-472 (2012-12-01)
Studies of protein dynamics, structure and interactions using hydrogen/deuterium exchange mass spectrometry (HDX-MS) have sharply increased over the past 5-10 years. The predominant technology requires fast digestion at pH 2-3 to retain deuterium label. Pepsin is used almost exclusively, but
Minghuo Wu et al.
Chemical communications (Cambridge, England), 49(14), 1407-1409 (2013-01-17)
A simple, novel "one-pot" approach was developed for the preparation of organic-silica hybrid monoliths. Two clickable monoliths functionalized with either azido or alkynyl moieties were prepared. The clickable monoliths were further used for the fabrication of enzyme reactors via "click
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持