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产品名称
Discovery® HS C18 高效液相色谱柱, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L1
产品线
Discovery®
特点
endcapped
制造商/商品名称
Discovery®
包装
1 ea of
标记范围
20% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
15 cm × 2.1 mm
表面积
300 m2/g
表面覆盖度
3.2 μmol/m2
杂质
<10 ppm metals
基质
silica gel, high purity, spherical particle platform
fully porous particle
基质活性基团
C18 (octadecyl) phase
粒径
5 μm
孔径
120 Å
工作pH范围
2-8
应用
food and beverages
分离技术
reversed phase
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商品
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
相关内容
HPLC DiscoveryBIO HS C18 products. Available in 3µm, 5µm and 10µm particle sizes.
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