Anti-PPARδ Antibody, clone 1I8 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1I8 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects PPAR . It targets an epitope within 21 amino acids from the N-terminal half.

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human PPAR .

Quality Control Testing

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PPAR in human heart tissue lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PPAR in HT-1080 cells.

Affinity Binding Assay: A representative lot of this antibody bound PPAR peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Peroxisome proliferator-activated receptor delta (UniProt: Q03181 also known as PPAR-delta, NUCI,Nuclear hormone receptor 1, NUC1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, PPAR-beta) is encoded by the PPARD (also known as NR1C2, PPARB) gene (Gene ID: 5467) in human. PPARD is a member of the peroxisome proliferator-activated receptor (PPAR) family. It is a ligand-activated transcription factor, which is a key mediator of energy metabolism in adipose tissues. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR delta and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The protein is ubiquitously expressed with maximal levels in placenta, skeletal muscle and adipose tissue. PPARD is primarily localized in the nucleus. It has a modular structure composed of several domains: a DNA-binding domain (DBD) with two zinc finger motifs, a hinge region, and a ligand-binding domain (LBD). Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Dysregulation of PPAR-Delta activity has been linked to obesity, type 2 diabetes, dyslipidemia, and cardiovascular diseases. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.