Anti-TUBB6 Antibody, clone 1F12 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1F12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Tubulin beta-6 chain (TUBB6). It targets an epitope within 20 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Tubulin beta-6 chain (TUBB6).

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TUBB6 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TUBB6 in MDA-MB-231, U251-MG, NIH3T3, and PC12 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TUBB6 in Human tonsil and Human fallopian tube tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TUBB6 in A431, HeLa, HUVEC, and NIH 3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound TUBB6 peptide with a KD of 3.9 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Tubulin beta-6 chain (UniProt: Q9BUF5; also known as TUBB6, Tubulin beta class V) is encoded by the TUBB6 gene (Gene ID: 84617) in human. Tubulin is the major constituent of microtubules (MTs). MTs are composed of α/β-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. α/β heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the β-tubulin subunit facing the microtubule plus end conferring a structural polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the α-chain. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. β-Tubulin is reported to undergo phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. TUBB6 is ubiquitously distributed with maximal expression observed in breast and lung, where it represents around 10% of all β-tubulins. High expression of TUBB6 is also observed in osteoclasts and is important for bone resorption.  Its expression is largely decreased in most cancerous tissues. In malignant transformation TUBB6 has been associated with cancer invasion and metastasis in several types of aggressive malignancies. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, B., et al (2023). Int. J. Cancer. 152(2); 32-330; Maurin, J., et al. (2021). Front. Cell Dev. Biol. 9; 778887; Fourest-Lieuvin, A., et al. (2006). Mol. Cell Biol. 17(3); 1041-1050).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.