Anti-DNMT1 Antibody, clone 1L14 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1L14 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects DNA (cytosine-5)-methyltransferase 1 (DNMT1). It targets an epitope within 23 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of human DNA (cytosine-5)-methyltransferase 1 (DNMT1).

Quality Control Testing

Evaluated by Western Blotting in K562 cell nuclear extract.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected DNMT1 in K562 cell nuclear extract.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from representative lot detected DNMT1 in HeLa and NTERA-2 cell nuclear extracts and recombinant human DNMT1.

Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected DNMT1 in HeLa cells.

Affinity Binding Assay: A representative lot of this antibody bound DNMT1 with a KD of 1.1 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from representative lot detected DNMT1 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

DNA (cytosine-5)-methyltransferase 1 (UniProt: P26358; also known as EC: 2.1.1.37, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT) is encoded by the DNMT1 (also known as AIM, CXXC9, DNMT) gene (Gene ID: 1786) in human. DNMT1 is an important epigenetic regulator that plays a key role in the maintenance of DNA methylation. It is a homodimeric protein that is ubiquitously expressed. Higher expression of DNMT1 is observed in fetal tissues and in heart, kidney, placenta, and peripheral mononuclear cells. Its abundance is reduced to almost non-detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Its expression is regulated by many signal transduction pathways, including PI3/Akt, Rb/E2F and p53/SP1. DNMT1 is shown to preferentially methylate hemimethylated DNA and it associates with DNA replication sites in the S phase to maintain the methylation pattern in newly synthesized strand, which is essential for epigenetic inheritance. DNMT1 associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is also responsible for maintaining methylation patterns established in development. It is reported to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells. DNMT1 can undergo acetylation on multiple lysine residues mainly by KAT2B/PCAF. It can be phosphorylated on Serine 154 by Cdks and this phosphorylation is considered to be essential for its enzymatic activity and protein stability. Its phosphorylation on serine 143 by Akt1 is reported to prevent methylation by SETD7, which increases its stability. Mutations in DNMT1 gene are reported to cause hereditary sensory neuropathy that is characterized by adult onset of progressive peripheral sensory loss associated with progressive hearing impairment and early-onset dementia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cheng, J., et al. (2015), Nat. Commun. 6; 7023; Serra, RW., et al. (2014). Elife. 3; e02313).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.