ZRB1639
Anti-TrkA Antibody, clone 2J4 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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High affinity nerve growth factor receptor;EC:2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;gp140trk;p140-TrkA
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生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2J4, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt 87.5 kDa
observed mol wt ~140 kDa
纯化方式
using Protein A
种属反应性
human
种属反应性(根据同源性预测)
monkey
包装
antibody small pack of 25 μL
增强验证
recombinant expression
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sustainability
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技术
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
C-terminal
Protein ID登记号
UniProt登记号
环保替代产品分类
, Aligned
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... NTRK1(4914)
一般描述
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2J4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects TrkA . It targets an epitope within 21 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal, cytoplasmic domain of human TrkA.
应用
Quality Control Testing
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TrkA in K562 cell lysate.
Tested Applications
Western Blotting Analysis (WB): 1:10,000 dilution of this antibody detected His-tagged recombinant fragment of Human TrkA.
Affinity Binding Assay: A representative lot of this antibody bound TrkA peptide with a KD of 4.3 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in SH-SY5Y cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TrkA in human adrenal gland tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TrkA in K562 cell lysate.
Tested Applications
Western Blotting Analysis (WB): 1:10,000 dilution of this antibody detected His-tagged recombinant fragment of Human TrkA.
Affinity Binding Assay: A representative lot of this antibody bound TrkA peptide with a KD of 4.3 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in SH-SY5Y cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TrkA in human adrenal gland tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
目标描述
High affinity nerve growth factor receptor (UniProt: P04629; also known as EC:2.7.10.1, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA) is encoded by the NTRK1 (also known as MTC, TRK, TRKA) gene (Gene ID: 4914) in human. TrkA is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 33-423), a transmembrane domain (aa 424-439), and a cytoplasmic domain (aa 440-796). The extracellular domain of Trk-A is composed by five domains: a leucine-rich region, two cysteine-rich domains and two immunoglobulin (Ig)-like domains. TrkA serves as a receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous system through regulation of proliferation, differentiation, and survival of sympathetic and nervous neurons. It serves as a high affinity receptor for NGF, which is its primary ligand. It can also bind and be activated by neurotrophin-3 (NTF3). However, NTF3 only supports axonal extension through NTRK1 but does not affect neuron survival. Upon dimeric NGF ligand-binding, it undergoes homodimerization, autophosphorylation. and activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wehrman, T., et al. (2007). Neuron. 53(1); 25-38; Covaceuszach, S., et al. (2005). Proteins. 58(3); :717-727; Jing, S., et al. (1992). Neuron. 9(6); 1067-1079).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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