推荐产品
一般描述
蛋白质组学动态范围标准品套装由48种单独的人源或人序列重组蛋白的混合物产生，每种重组蛋白都被选择用于限制异源翻译后修饰（PTM）。蛋白质标准品由6种蛋白质的6种混合物配制而成，其动态范围为5个数量级，范围为50皮摩尔至500摩尔。在配制之前，通过氨基酸分析（AAA）对每种蛋白质进行定量。
应用
蛋白组学动态范围标准套件用于基于强度的绝对定量（iBAQ），适用 大肠杆菌 蛋白、 胚胎干细胞（ESC）和神经元前体细胞（NPC）蛋白组。 也作为标准品，无标记定量刺突 HeLa 细胞。
蛋白质组学动态范围标准品套装可用于在分析复杂蛋白质样品之前标准化和/或评估质谱（例如，LC-MS / MS，MALDI-TOF-MS等）和电泳分析条件。UPS2 可用于在已知复杂标准样品的运行之间交叉包含宝贵的实验数据集。从而确认分析方法的耐用性和所用仪器的稳定性。此外，生成或比较来自定义不明确的样品的质谱数据的实验室可以使用UPS2作为外部参考，以协助评估结果和实验方法。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS（液相色谱 - 质谱）/ MS分析中基于强度的蛋白质绝对定量（iBAQ）的标准。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS（液相色谱 - 质谱）/ MS分析中基于强度的蛋白质绝对定量（iBAQ）的标准。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg化学品安全说明书
警示用语：
Danger
危险分类
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
Christina Ludwig et al.
Molecular & cellular proteomics : MCP, 11(3), M111-M111 (2011-11-22)
For many research questions in modern molecular and systems biology, information about absolute protein quantities is imperative. This information includes, for example, kinetic modeling of processes, protein turnover determinations, stoichiometric investigations of protein complexes, or quantitative comparisons of different proteins
Jacek R Wiśniewski et al.
Molecular systems biology, 8, 611-611 (2012-09-13)
We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying > 7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal and cancer tissues
Rosemary Yu et al.
Nature communications, 11(1), 1881-1881 (2020-04-22)
Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and
Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A, et al.
Molecular and Cellular Proteomics, 13, 1925-1925 (2014)
Lee Dicker et al.
Molecular & cellular proteomics : MCP, 9(12), 2704-2718 (2010-09-09)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics provides a wealth of information about proteins present in biological samples. In bottom-up LC-MS/MS-based proteomics, proteins are enzymatically digested into peptides prior to query by LC-MS/MS. Thus, the information directly available from the LC-MS/MS
商品
High-throughput proteomics advances with improved analysis methods and mass spectrometry.
相关内容
Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.
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