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重组
expressed in baculovirus
质量水平
表单
buffered aqueous glycerol solution
分子量
100 kDa
浓度
≥2 units/μL
UniProt登记号
储存温度
−70°C
基因信息
human ... TOP1(7150)
一般描述
Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.
应用
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
生化/生理作用
Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.
单位定义
One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.
外形
Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).
警示用语：
Danger
危险声明
预防措施声明
危险分类
Repr. 1B
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Binding and dissociation of human topoisomerase I with hairpin-loop RNAs: implications for the regulation of HIV-1 replication
Takahashi H, Sawa H, Hasegawa H,
Biochemical and Biophysical Research Communications, 297(3), 593-599 (2012)
M Lisby et al.
The Journal of biological chemistry, 276(23), 20220-20227 (2001-04-03)
All eukaryotic forms of DNA topoisomerase I contain an extensive and highly charged N-terminal domain. This domain contains several nuclear localization sequences and is essential for in vivo function of the enzyme. However, so far no direct function of the
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA
Redinbo MR, et al.
Biochemistry, 39(23), 6832-6840 (2000)
Molecular Networking Reveals Two Distinct Chemotypes in Pyrroloiminoquinone-Producing Tsitsikamma favus Sponges
Kalinski JCJ, et al.
Marine drugs, 17(1), 60-60 (2019)
Mechanisms of topoisomerase I (TOP1) gene copy number increase in a stage III colorectal cancer patient cohort
Smith DH, et al.
Testing, 8(4), e60613-e60613 (2013)
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