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重组
expressed in baculovirus
质量水平
表单
buffered aqueous glycerol solution
分子量
100 kDa
浓度
≥2 units/μL
UniProt登记号
储存温度
−70°C
基因信息
human ... TOP1(7150)
一般描述
Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.
应用
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
生化/生理作用
Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.
单位定义
One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.
外形
Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).
警示用语：
Danger
危险声明
预防措施声明
危险分类
Repr. 1B
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Binding and dissociation of human topoisomerase I with hairpin-loop RNAs: implications for the regulation of HIV-1 replication
Takahashi H, Sawa H, Hasegawa H,
Biochemical and Biophysical Research Communications, 297(3), 593-599 (2012)
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA
Redinbo MR, et al.
Biochemistry, 39(23), 6832-6840 (2000)
M Lisby et al.
The Journal of biological chemistry, 276(23), 20220-20227 (2001-04-03)
All eukaryotic forms of DNA topoisomerase I contain an extensive and highly charged N-terminal domain. This domain contains several nuclear localization sequences and is essential for in vivo function of the enzyme. However, so far no direct function of the
Mechanisms of topoisomerase I (TOP1) gene copy number increase in a stage III colorectal cancer patient cohort
Smith DH, et al.
Testing, 8(4), e60613-e60613 (2013)
Molecular Networking Reveals Two Distinct Chemotypes in Pyrroloiminoquinone-Producing Tsitsikamma favus Sponges
Kalinski JCJ, et al.
Marine drugs, 17(1), 60-60 (2019)
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