生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
TUB-1A2, monoclonal
包含
15 mM sodium azide
种属反应性
human, plant, animal
技术
indirect immunofluorescence: 1:800 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable
同位素/亚型
IgG3
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TUBA4A(7277) , TUBB(203068)
一般描述
单克隆抗络氨酸微管蛋白(小鼠 IgM 同种型)来源于杂交瘤,该杂交瘤由小鼠骨髓瘤细胞与免疫小鼠脾细胞融合产生。微管蛋白为圆柱形丝状结构,几乎存在于所有真核细胞中。微管蛋白是异二聚体,由 α-微管蛋白和 β-微管蛋白组成。 每种亚基的分子量均为55 kDa,具有相当大的同源性。
微管,是细胞内圆柱状丝状结构,主要由微管蛋白组成,存在于所有真核细胞中。单克隆抗酪氨酸抗体可通过间接免疫荧光标记,对酪氨酸 α 微管蛋白进行免疫细胞化学定位。 单克隆抗酪氨酸抗体与酪氨酸微管蛋白发生特异性反应,适用于牛脑细胞、非洲绿猴肾细胞(Vero),犬肾细胞(MDCK)、袋鼠肾细胞(Potoroo,PtK2)、小鼠垂体瘤细胞(AtT-20)、酵母细胞和非洲爪蟾细胞。
特异性
该抗体在免疫印迹测定中与微管蛋白的C-末端酪氨酸反应,可用于培养细胞或组织切片中该表位的定位。
免疫原
含有 α-微管蛋白羧基端氨基酸的肽
应用
单克隆抗酪氨酸微管蛋白用于:
- 间接免疫荧光标记
- 免疫印迹技术
- 免疫细胞化学染色
生化/生理作用
微管蛋白是微管的主要构建单元。微管蛋白的组装过程涉及微管蛋白酪氨酸化。特异性微管蛋白酪氨酸羧肽酶去除末端酪氨酸,产生末端为谷氨酸残基的 α-微管蛋白;而另一种酶通过在羧基末端添加酪氨酸修饰 α-微管蛋白,从而形成酪氨酸增加和损失的潜在循环。
免责声明
除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
Not finding the right product?
Try our 产品选型工具.
WGK
WGK 1
法规信息
常规特殊物品
The Journal of general physiology, 146(1), 109-117 (2015-07-01)
A set of bipolar cells in the retina of goldfish contains giant synaptic terminals that can be over 10 µm in diameter. Hundreds of thousands of synaptic vesicles fill these terminals and engage in continuous rounds of exocytosis. How the
BMC biology, 12, 26-26 (2014-04-01)
The evolutionary origin of gastrulation--defined as a morphogenetic event that leads to the establishment of germ layers--remains a vexing question. Central to this debate is the evolutionary relationship between the cell layers of sponges (poriferans) and eumetazoan germ layers. Despite
Microtubule Structures
Molecular Cell Biology. 4th edition. (2000)
Journal of cellular biochemistry, 81(1), 162-171 (2001-02-17)
Conflicting data for the effects of colchicine on cholesterol transport and steroidogenesis raise the question of the role of microtubules in cholesterol transport from the lipid droplet to mitochondria in steroidogenic cells. In this study, using corticosterone radioimmunoassay and immunofluorescence
The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica.
EvoDevo, 7, 6-6 (2016-03-10)
The aquiferous body plan of poriferans revolves around internal chambers comprised of choanocytes, a cell type structurally similar to choanoflagellates. These choanocyte chambers perform a range of physiological and developmental functions, including the capture of food and the generation of
商品
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门