Monoclonal Anti-β-Tubulin I+II antibody produced in mouse

β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with a core of two β-sheets enclosed by α-helices. In addition, it also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. β-tubulin I and II is encoded by the gene mapped to human chromosome 20 and 6p25.2 respectively.

Monoclonal Anti-β-Tubulin Isotype I + II (mouse IgG2b isotype) is derived from the JDR.3B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

The antibody specifically recognizes an epitope located on isotypes I and II of β-tubulin. No cross-reactivity with other tubulin isotypes is observed.

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype II coupled to BSA

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in following studies:

• immunoblot
• immunofluorescence technique
• immunohistochemistry.

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunohistochemistry
• western blotting
• immunoblotting
• dot blot
• immunocytochemistry

β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Tubulin β 2B class IIb (TUBB2B)/β-tubulin II plays a vital role in migration/organization and axon guidance processes. Mutation in β-tubulin gene is associated with the development of asymmetric polymicrogyria (PMG).

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