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Merck
CN

T8453

Sigma-Aldrich

Anti-β-Tubulin II antibody, Mouse monoclonal

clone 7B9, purified from hybridoma cell culture

别名:

Anti-TUBB2, Anti-Tubulin, β2

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

7B9, monoclonal

形式

buffered aqueous solution

分子量

antigen ~50 kDa

种属反应性

human, mouse, rat

浓度

~1.0 mg/mL

技术

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract

同位素/亚型

IgG1

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

一般描述

Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology.

应用

Anti-β-Tubulin II antibody has been used in
  • immunoblotting immunoprecipitation
  • immunocytochemistry
  • western blotting
  • immunolabelling

生化/生理作用

The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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访问文档库

Mutations affecting beta-tubulin folding and degradation
Wang Y, et al.
The Journal of Biological Chemistry, 281(19), 13628-13635 (2006)
Hongfeng Yuan et al.
Oncotarget, 8(9), 14912-14924 (2017-01-25)
Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive non-Hodgkin lymphomas. It is curable but one-third of cases are refractory to therapy or relapse after initial response highlighting the urgent need for developing novel therapeutic approaches. Targeting sirtuins
Spatio-temporal dynamics of $\beta$-tubulin isotypes during the development of the sensory auditory organ in rat
Renauld J, et al.
Histochemistry and Cell Biology, 144(5), 403-416 (2015)
FOXP1 potentiates Wnt/ beta-catenin signaling in diffuse large B cell lymphoma
Walker M P, et al.
Science Signaling, 8(362), ra12-ra12 (2015)
Salama A Salama et al.
Fertility and sterility, 98(1), 178-184 (2012-05-15)
To investigate the effects and the mechanism of action of 2-methoxyestradiol (2ME(2)) on transforming growth factor (TGF) β3-induced profibrotic response in immortalized human uterine fibroid smooth muscle (huLM) cells. Laboratory study. University research laboratory. Not applicable. Not applicable. huLM cells

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