生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
7B9, monoclonal
形式
buffered aqueous solution
分子量
antigen ~50 kDa
种属反应性
human, mouse, rat
浓度
~1.0 mg/mL
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TUBB2B(347733)
mouse ... Tubb2b(73710)
rat ... Tubb2b(291081)
一般描述
Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology.
应用
Anti-β-Tubulin II antibody has been used in
- immunoblotting immunoprecipitation
- immunocytochemistry
- western blotting
- immunolabelling
生化/生理作用
The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Mutations affecting beta-tubulin folding and degradation
The Journal of Biological Chemistry, 281(19), 13628-13635 (2006)
Oncotarget, 8(9), 14912-14924 (2017-01-25)
Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive non-Hodgkin lymphomas. It is curable but one-third of cases are refractory to therapy or relapse after initial response highlighting the urgent need for developing novel therapeutic approaches. Targeting sirtuins
Spatio-temporal dynamics of $\beta$-tubulin isotypes during the development of the sensory auditory organ in rat
Histochemistry and Cell Biology, 144(5), 403-416 (2015)
FOXP1 potentiates Wnt/ beta-catenin signaling in diffuse large B cell lymphoma
Science Signaling, 8(362), ra12-ra12 (2015)
Fertility and sterility, 98(1), 178-184 (2012-05-15)
To investigate the effects and the mechanism of action of 2-methoxyestradiol (2ME(2)) on transforming growth factor (TGF) β3-induced profibrotic response in immortalized human uterine fibroid smooth muscle (huLM) cells. Laboratory study. University research laboratory. Not applicable. Not applicable. huLM cells
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