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Merck
CN
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文件

安全信息

T8158

Sigma-Aldrich

转铁蛋白 人

powder, BioReagent, suitable for cell culture

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别名:
转铁蛋白,部分饱和
CAS号:
11096-37-0
EC 号:
234-318-8
MDL编号:
MFCD00132442
UNSPSC代码:
12352202
NACRES:
NA.75

产品线

BioReagent

质量水平

200

检测方案

≥98%

形式

powder

浓度

~25 mM

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested
 hepatitis and HIV antibody, none detected

溶解性

H2O: 50 mg/mL

UniProt登记号

P02787

运输

ambient

储存温度

−20°C

基因信息

human ... TF(7018)

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相关类别

一般描述

转铁蛋白是具有分支糖链的糖蛋白，多肽链中具有两个铁离子结合位点。

应用

人转铁蛋白已用于：
  • 与IgG一起孵育人近端肾小管细胞 (HKC)-8个细胞，检验白蛋白特异性
  • 用作人糖原细胞培养的含血清/无人甲状腺细胞培养液的补充剂
  • 用作开发凝胶肼解法的靶糖蛋白

生化/生理作用

转铁蛋白介导铁从血浆向细胞的运输。与铁结合的转铁蛋白具有三种形式：单铁A/B转铁蛋白和二铁转铁蛋白。转铁蛋白可与特定的细胞表面受体结合，通过内吞作用内化。进入细胞后，铁被释放到细胞中，无铁转铁蛋白（也称为脱铁转铁蛋白）则通过胞吐作用释放到细胞外，而不会被降解。目前确知转铁蛋白可将铁以生理学安全有效的形式递送至细胞。

其他说明

部分铁饱和

制备说明

60℃热处理10小时。

分析说明

通过琼脂糖电泳纯化。

免责声明

供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Non-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot P, et al.
Biochim. Biophys. Acta Gen. Subj., 1820(3), 403-410 (2012)
Martijn F Hoes et al.
European journal of heart failure, 20(5), 910-919 (2018-02-28)
Iron deficiency is common in patients with heart failure and associated with a poor cardiac function and higher mortality. How iron deficiency impairs cardiac function on a cellular level in the human setting is unknown. This study aims to determine
Cells: Molecules and Mechanisms, 174-174 (2009)
Glucose-6-phosphate dehydrogenase activity in monolayer cultures of thyroid epithelial cells: TSH and inhibition of nitrogen oxide synthase affect the enzyme activity and the oxygen sensitivity of the histochemical assay
Kayser L and Thomsen J
Acta Histochemica, 107(1), 31-41 (2005)
A Dautry-Varsat et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(8), 2258-2262 (1983-04-01)
At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to the same extent and with the same affinity as does diferric transferrin at pH 7.0. Apotransferrin is quickly dissociated from its receptor when the pH is raised to
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