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生物来源
human pancreas
质量水平
形式
salt-free, lyophilized powder
包装
vial of ≥1,000 BAEE units
储存温度
−20°C
基因信息
human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)
相关类别
一般描述
胰蛋白酶分子有两个结构域：一个与酶的活性位点和色氨酸残基有关；另一种与 8-苯胺萘-1-磺酸结合有关。
胰蛋白酶是一种丝氨酸蛋白酶。胰腺可产生前体胰蛋白酶原并将其分泌到肠中。肠粘膜中的肠激酶可将胰蛋白酶原转化为胰蛋白酶。
应用
胰蛋白酶已被用于一项评估戊型肝炎病毒和人类星状病毒之间的相似性的研究。在一项研究中也使用了胰蛋白酶来表征培养原代成人上皮祖细胞或干细胞的一种独特技术。
单位定义
使用BAEE作为底物，一个BAEE单元将在pH7.6,25℃下产生每分钟0.001的A253。 反应体积= 3.2mL（1cm光程）。
数量
至少 1,000 个 BAEE 单位/瓶
分析说明
通过 E1%/280 确定的蛋白质。
其他说明
查看更多关于 胰蛋白酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
底物
抑制剂
产品编号
说明
价格
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
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实验方案
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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