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生物来源
human pancreas
质量水平
表单
salt-free, lyophilized powder
包装
vial of ≥1,000 BAEE units
储存温度
−20°C
基因信息
human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)
相关类别
一般描述
胰蛋白酶分子有两个结构域：一个与酶的活性位点和色氨酸残基有关；另一种与 8-苯胺萘-1-磺酸结合有关。
胰蛋白酶是一种丝氨酸蛋白酶。胰腺可产生前体胰蛋白酶原并将其分泌到肠中。肠粘膜中的肠激酶可将胰蛋白酶原转化为胰蛋白酶。
应用
胰蛋白酶已被用于一项评估戊型肝炎病毒和人类星状病毒之间的相似性的研究。在一项研究中也使用了胰蛋白酶来表征培养原代成人上皮祖细胞或干细胞的一种独特技术。
单位定义
使用BAEE作为底物，一个BAEE单元将在pH7.6,25℃下产生每分钟0.001的A253。 反应体积= 3.2mL（1cm光程）。
数量
至少 1,000 个 BAEE 单位/瓶
分析说明
通过 E1%/280 确定的蛋白质。
其他说明
查看更多关于 胰蛋白酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
底物
抑制剂
产品编号
说明
价格
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Kelly A Dryden et al.
Journal of molecular biology, 422(5), 650-658 (2012-06-30)
Human astroviruses (HAstVs) are a major cause of gastroenteritis. HAstV assembles from the structural protein VP90 and undergoes a cascade of proteolytic cleavages. Cleavage to VP70 is required for release of immature particles from cells, and subsequent cleavage by trypsin
Jia Liu et al.
Nature protocols, 16(7), 3522-3546 (2021-06-06)
Cellular heterogeneity is pervasive and of paramount importance in biology. Single-cell analysis techniques are indispensable for understanding the heterogeneity and functions of cells. Low-copy-number proteins (fewer than 1,000 molecules per cell) perform multiple crucial functions such as gene expression, cellular
Industrial Enzymes and Their Applications (1998)
Cynthia Luz Marcelo et al.
BMC dermatology, 12, 8-8 (2012-06-26)
Primary keratinocytes derived from epidermis, oral mucosa, and urothelium are used in construction of cell based wound healing devices and in regenerative medicine. This study presents in vitro technology that rapidly expands keratinocytes in culture by growing monolayers under large
Mian Zhou et al.
Nature, 495(7439), 111-115 (2013-02-19)
Codon-usage bias has been observed in almost all genomes and is thought to result from selection for efficient and accurate translation of highly expressed genes. Codon usage is also implicated in the control of transcription, splicing and RNA structure. Many
实验方案
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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