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Merck
CN

T6400

Tris-硼酸-EDTA缓冲溶液

5× Concentrate

别名:

TBE 缓冲液

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
41105319
MDL number:
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InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

sterility

0.2 μm filtered

form

solution

impurities

DNase and RNase, none detected

pH

8.2-8.4 (25 °C)

Quality Level

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Application

TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性(7M 尿素)凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶,但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液,生成的缓冲液含有89mM Tris-硼酸和2mM EDTA,pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液,用来制成PAGE胶。 为了达到最大的分辨率,推荐电压小于5V/cm(电极单元间距离)。
Tris-Borate-EDTA 缓冲液已用于电泳分离从活性污泥中提取的质粒。
适用于稀释至使用浓度后的凝胶电泳。

Other Notes

0.445M Tris 硼酸,pH 约为 8.3,含有 0.01M EDTA。

Packaging

用带龙头分液器包装。

Preparation Note

用 18 兆欧水制备的溶液
采用经生物技术性能认证的 Trizma 碱(产品代码 T6066)、分子生物学试剂硼酸(产品代码 B6768)和 EDTA 二钠盐(产品代码 E5134)制备。

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Bauda P, et al.
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Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
Seung-min Park et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15549-15554 (2009-09-01)
Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques

实验方案

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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