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文件

T6400

Tris-硼酸-EDTA缓冲溶液

Name: Tris-硼酸-EDTA缓冲溶液
Brand: SIGMA

5× Concentrate

别名:

TBE 缓冲液

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About This Item

MDL编号:

MFCD00236358

UNSPSC代码:

41105319

PubChem化学物质编号:

329826638

NACRES:

NA.25

质量水平

200

无菌性

0.2 μm filtered

形式

solution

杂质

DNase and RNase, none detected

pH值（酸碱度）

8.2-8.4 (25 °C)

SMILES字符串

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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应用

Tris-Borate-EDTA 缓冲液已用于电泳分离从活性污泥中提取的质粒。

适用于稀释至使用浓度后的凝胶电泳。

TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

包装

用带龙头分液器包装。

其他说明

0.445M Tris 硼酸，pH 约为 8.3，含有 0.01M EDTA。

制备说明

用 18 兆欧水制备的溶液

采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

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