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生物来源
Clostridium tetani E88
分子量
55 kDa
应用
cell analysis
储存温度
2-8°C
基因信息
Clostridium tetani E88 ... CTC01888(1060232)
一般描述
Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.
应用
Tetanolysin has been used to analyze the formation of lytic pores in red blood cells (RBCs)1. It has also been used to permeabilize infected RBCs2.
生化/生理作用
Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.
制备说明
Prepared by a modification of the method of Haque, et al.
重悬
When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.
分析说明
Single band by SDS-PAGE.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Hayley E Bullen et al.
The Journal of biological chemistry, 287(11), 7871-7884 (2012-01-19)
To survive within its host erythrocyte, Plasmodium falciparum must export hundreds of proteins across both its parasite plasma membrane and surrounding parasitophorous vacuole membrane, most of which are likely to use a protein complex known as PTEX (Plasmodium translocon of
Jason W Rosch et al.
The Journal of clinical investigation, 120(2), 627-635 (2010-01-23)
Sickle cell disease (SCD) is characterized by intravascular hemolysis and inflammation coupled to a 400-fold greater incidence of invasive pneumococcal infection resulting in fulminant, lethal pneumococcal sepsis. Mechanistically, invasive infection is facilitated by a proinflammatory state that enhances receptor-mediated endocytosis
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