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Merck
CN
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文件

T4415

Sigma-Aldrich

Tris-硼酸-EDTA缓冲溶液

BioReagent, suitable for electrophoresis, 10× concentrate

别名:

TBE 缓冲液

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About This Item

MDL编号:
MFCD00236358
UNSPSC代码:
41105319
PubChem化学物质编号:
329826793
NACRES:
NA.25

质量水平

200

产品线

BioReagent

形式

solution

杂质

DNase and RNase, none detected

适用性

suitable for electrophoresis

SMILES字符串

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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相关类别

一般描述

TBE（Tris/硼酸/EDTA)缓冲液通常用于核酸电泳。该溶液在弱碱性条件下有效，能够保持DNA去质子化，水溶性，免于降解。该浓缩溶液能够方便地在使用前稀释到1x或0.5x（分子生物级别水）。

应用

稀释到工作浓度后，可用于凝胶电泳。
Tris-硼酸-EDTA缓冲液用于脉冲电场凝胶电泳。
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

包装

4L、10L 和 20L 容量用带龙头分液器包装。

制备说明

用 18 兆欧水制备
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

其他说明

TBE 缓冲剂会逐渐沉淀。通常而言，沉淀不会影响性能。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H360FD

危险分类

Repr. 1B

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

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L Orbán et al.
Electrophoresis, 12(4), 233-240 (1991-04-01)
DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. Polyacrylamide gels at pH 8.9, 2 degrees
M Wu et al.
BioTechniques, 24(4), 676-678 (1998-06-13)
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose
Characterization of a Clavibacter michiganensis subsp. michiganensis population in Israel.
Kleitman, F., Barash, I., Burger, A., Iraki, N.,
European Journal of Plant Pathology, 121, 463-475 (2008)
Yaroslav A Kainov et al.
Nature communications, 11(1), 361-361 (2020-01-19)
Eukaryotic gene expression relies on extensive crosstalk between transcription and RNA processing. Changes in this composite regulation network may provide an important means for shaping cell type-specific transcriptomes. Here we show that the RNA-associated protein Srrt/Ars2 sustains embryonic stem cell
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
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