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Merck
CN

T4132

Sigma-Aldrich

全转铁蛋白 人

≥98%

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别名:
嗜铁蛋白,铁饱和
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.61

生物来源

human

质量水平

检测方案

≥98%

形式

powder

铁含量

1100-1600 μg/g

技术

cell culture | mammalian: suitable

杂质

HIV, hepatitis B and hepatitis C, none detected

UniProt登记号

储存温度

2-8°C

基因信息

human ... TF(7018)

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相关类别

一般描述

转铁蛋白(TF)属于双环糖蛋白家族,其可结合三价铁。蛋白质的同源N-和C-叶具有存在于深隙中的单个铁结合位点。编码TF的基因位于人染色体3q22.1上。

应用

人全转铁蛋白已被用于:
  • 菌落形成检测
  • 转染的HEK-293(人胚肾)细胞或骨髓细胞中转铁蛋白的摄取和再循环
  • 制备促黄体化和非黄体化的颗粒细胞

生化/生理作用

研究表明,用全转铁蛋白(其可增加细胞内的铁水平和青蒿素衍生物)共同处理乳腺癌细胞会增加细胞的死亡。
转铁蛋白(TF)是血液中的铁转运蛋白。铁通过与循环转铁蛋白结合而在血清中进行转运,转铁蛋白又可与细胞表面上的受体进行结合。在碱性细胞外pH7.4的条件下,TF可结合一个或两个铁离子。铁结合的TF分子可与二聚体转铁蛋白受体(TfR)结合。在该pH下,TfR无法识别无铁转铁蛋白。随后便为TfR的内吞途径,其中整个复合物通过胞吞作用进入细胞内。随着细胞中pH的降低,铁会从TF中释放出来。然后复合物返回细胞表面,apo-TF分子与受体分离。

分析说明

经琼脂糖凝胶电泳纯度。

免责声明

供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

WGK

WGK 3

法规信息

常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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