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Merck
CN
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文件

T3913

Sigma-Aldrich

Tris-硼酸-EDTA缓冲溶液

5× concentrate, powder blend

别名:

TBE 缓冲液

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About This Item

MDL编号:
MFCD00236358
UNSPSC代码:
41105319
PubChem化学物质编号:
329826779
NACRES:
NA.25

质量水平

200

形式

powder blend

技术

electrophoresis: suitable

杂质

DNase, RNase and NICKase, none detected

pH值（酸碱度）

8.1-8.5 (25 °C, 5 ×)

溶解性

water: 85.1 g/L, clear, colorless

适用性

suitable for gel electrophoresis (after dilution to working concentration)

应用

diagnostic assay manufacturing

SMILES字符串

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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应用

Tris-硼酸-EDTA缓冲液适用于凝胶电泳。
它可用于以下研究：
  • 作为通过十二烷基硫酸钠-孔梯度电泳评估卵白蛋白、抗生物素蛋白和胎球蛋白糖蛋白分子量的缓冲系统。
  • 用于淀粉凝胶中的血红蛋白电泳。
  • 制备用于快速测定DNA序列的缓冲液浓度梯度凝胶。
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

包装

袋装

制备说明

采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

重悬

溶解于指定量的水中得到 5× 浓缩液（0.445M Tris-硼酸、10mM EDTA，pH 为 8.3）。须提供合适的容器。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H360FD

危险分类

Repr. 1B

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

分析证书(COA)

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THE AMINO ACID COMPOSITION OF HEMOGLOBIN. V. THE PREPARATION OF PURIFIED HEMOGLOBIN FRACTIONS BY CHROMATOGRAPHY ON CELLULOSE EXCHANGERS AND THEIR IDENTIFICATION BY STARCH GEL ELECTROPHORESIS USING TRIS-BORATE-EDTA BUFFER.
A I CHERNOFF et al.
Blood, 25, 646-661 (1965-05-01)
M D Biggin et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(13), 3963-3965 (1983-07-01)
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation
Glycoprotein molecular-weight estimation using sodium dodecyl sulfate-pore gradient electrophoresis: comparison of tris-glycine and tris-borate-EDTA buffer systems.
J F Poduslo
Analytical biochemistry, 114(1), 131-139 (1981-06-01)
Xiang Li et al.
Nucleic acids research, 47(10), 5074-5085 (2019-06-05)
In microorganisms, a number of metalloproteins including PerR are found to regulate gene expression in response to environmental reactive oxygen species (ROS) changes. However, discovery of similar regulatory mechanisms remains elusive within mammalian cells. As an antioxidant metalloenzyme that maintains
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
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