SRP5009
CDK1/CyclinB1, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
CCNB, CCNB1, CDC2
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
12-16 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
产品名称
CDK1/CyclinB1, active, GST tagged human, PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
12-16 nmol/min·mg
mol wt
~58 kDa (CDK1)
~75 kDa (CyclinB1)
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCNB1(891), CDK1(983)
Biochem/physiol Actions
The cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex plays a crucial role in the regulation of mitosis. Elevated expression of CCNB1 has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines.In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positive (ER+) breast cancer. CDK1 (cyclin-dependent kinase 1) controls various mitotic processes like reorganization of the cytoskeleton and segregation of chromosomes. It is essential for the multiplication of mammalian cells.
General description
The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells. CDK1 (cyclin-dependent kinase 1) belongs to the Ser/Thr protein kinase family. The gene encoding it is localized on human chromosome 10q21.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Importance of tuberin in carcinogenesis (Review)
Mingwei Jin
Oncology Letters (2017)
Recurrent CDK1 overexpression in laryngeal squamous cell carcinoma
K. Bednarek
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 37(8) (2016)
Soraya Játiva et al.
Cellular and molecular life sciences : CMLS, 76(18), 3601-3620 (2019-03-31)
Exit from mitosis and completion of cytokinesis require the inactivation of mitotic cyclin-dependent kinase (Cdk) activity. In budding yeast, Cdc14 phosphatase is a key mitotic regulator that is activated in anaphase to counteract Cdk activity. In metaphase, Cdc14 is kept
CCNB1 is a prognostic biomarker for ER+ breast cancer
Kun Ding
Medical Hypotheses, 83(3) (2014)
Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
Ivan B
Molecular Cancer (2011)
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