SRP3177
TNF-α 人
Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
别名:
分化诱导因子（DIF）, 坏死素, 恶病质素, 细胞毒素, 肿瘤坏死因子
产品名称
TNF-α 人, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized
potency
≤0.10 ng/mL ED50
mol wt
17.4 kDa
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg endotoxin
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TNF(7124)
Application
人TNF-α（肿瘤坏死因子α）已被用作诱导人肠和肝细胞系中促炎基因的阳性对照。
Biochem/physiol Actions
TNF-α（肿瘤坏死因子α）对多种肿瘤细胞具有细胞毒性，是介导免疫反应对抗细菌感染的重要因子。然而，它既可以是肿瘤促进剂，也可以是肿瘤抑制剂。TNF-α在感染性休克、自身免疫性疾病、类风湿性关节炎、炎症、细胞凋亡和糖尿病的诱导中也起作用。它还与情绪和焦虑症有关。
General description
TNF-α（肿瘤坏死因子α）是由多种细胞分泌的多效性促炎细胞因子，包括脂肪细胞，活化的单核细胞，巨噬细胞，B细胞，T细胞和成纤维细胞。它属于TNF配体家族，通过TNFR1和TNFR2两个受体进行信号传导。该基因定位于人类染色体6p21.3。重组人TNF-α是一种可溶性157个氨基酸的蛋白质（17.4 kDa），对应于全长跨膜蛋白的C末端胞外域。
Other Notes
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Physical form
从10mM磷酸钠，pH 7.4 + 0.5 mM DTT中冻干。
Preparation Note
在−20 °C下保存产品。复溶后，产品可在2-8 °C下储存长达1个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融。请勿储存于“无霜”冰箱中。
建议在复溶之前对西林瓶进行离心。可以在无菌水中复溶至0.1mg/mL的浓度来制备储备溶液。储备溶液可以用培养基进一步稀释。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Immunogenicity of Anti-TNF-alpha agents in autoimmune diseases.
Emi Aikawa N
Clinical Reviews in Allergy & Immunology, 38, 82-89 (2010)
Regulation of cytokines, cytokine inhibitors, and acute-phase proteins following anti-TNF-alpha therapy in rheumatoid arthritis.
Charles P
Journal of Immunology, 163, 1521-1528 (1999)
Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines.
McKim JM Jr
Food And Chemical Toxicology, 96, 1-10 (2016)
Breast cancer cells induce stromal fibroblasts to express MMP-9 via secretion of TNF-alpha and TGF-beta.
Stuelten CH
Journal of Cell Science, 118, 2143-2153 (2005)
Potential role of TNF-alpha in the pathogenesis of insulin resistance and type 2 diabetes.
Moller DE
Trends in Endocrinology and Metabolism, 11, 212-217 (2000)
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