biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
≤2.0 ng/mL ED50
mol wt
7.5 kDa
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
off-white to yellow
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IGF2(3481)
General description
Insulin-like growth factors (IGFs) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.
Application
IL-4 from mouse has been used to activate B-cells.
Biochem/physiol Actions
The insulin-like growth factors (IGFs) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
PAPP-A-Specific IGFBP-4 Proteolysis in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Adasheva, et al.
International Journal of Molecular Sciences, 24 (2023)
Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation
Kyong-RimKieffer-Kwon
Molecular Cell, 67(4), 566-578 (2017)
Role of IGF-I Signaling in Muscle Bone Interactions
Daniel D, et.al
Bone, 80, 79-88 (2015)
Role of IGF-I Signaling in Muscle Bone Interactions
Daniel D Bikle
Bone, 80, 79-88 (2015)
A J D'Ercole
Endocrinology and metabolism clinics of North America, 25(3), 573-590 (1996-09-01)
The IGFs are essential stimulators of fetal and postnatal growth. Their signals are mediated predominantly by the type I IGF receptor, but another as yet unidentified receptor seems to mediate some actions, especially some of those for IGF-II. During organogenesis
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