生物来源
human
重组
expressed in E. coli
检测方案
≥80% (SDS-PAGE)
形式
aqueous solution
分子量
59 kDa
包装
pkg of 20 μg
制造商/商品名称
Sigma-Aldrich
储存条件
avoid repeated freeze/thaw cycles
浓度
1.8 mg/mL
技术
cell based assay: suitable
溶解性
water: soluble
NCBI登记号
UniProt登记号
应用
life science and biopharma
运输
dry ice
储存温度
−70°C
基因信息
human ... PKM(5315)
一般描述
Research area: Cell Signaling
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
应用
PKM1active human has been used in:
- cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
- oxidative stress experiments on PKM2.
- Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).
生化/生理作用
Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
C Parkison et al.
Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)
Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and
Nuclear Pyruvate Kinase M2 (PKM2) Contributes to Phosphoserine Aminotransferase 1 (PSAT1)-Mediated Cell Migration in EGFR-Activated Lung Cancer Cells
Biyik-Sit R, et al.
Cancer, 13(16), 3938-3938 (2021)
Research progress on the role of PKM2 in the immune response
Chunyan L, et al.
Frontiers in Immunology (2022)
Development of Novel Irreversible Pyruvate Kinase M2 Inhibitors
I-Shan H, et al.
Journal of Medicinal Chemistry, 62(18), 8497-8510 (2019)
Evaluation of serum and pleural levels tumor M2-pyruvate kinase in lung cancer patients with pleural effusion
Tiantian Z, et al.
BMC Pulmonary Medicine, 22, 307-307 (2022)
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