SRP0415
PKM1 active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
别名:
OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
产品名称
PKM1 active human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
59 kDa
packaging
pkg of 20 μg
manufacturer/tradename
Sigma-Aldrich
storage condition
avoid repeated freeze/thaw cycles
concentration
1.8 mg/mL
technique(s)
cell based assay: suitable
solubility
water: soluble
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PKM(5315)
Application
PKM1active human has been used in:
- cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
- oxidative stress experiments on PKM2.
- Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).
Biochem/physiol Actions
Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.
General description
Research area: Cell Signaling
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Research progress on the role of PKM2 in the immune response
Chunyan L, et al.
Frontiers in Immunology (2022)
Nuclear Pyruvate Kinase M2 (PKM2) Contributes to Phosphoserine Aminotransferase 1 (PSAT1)-Mediated Cell Migration in EGFR-Activated Lung Cancer Cells
Biyik-Sit R, et al.
Cancer, 13(16), 3938-3938 (2021)
C Parkison et al.
Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)
Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and
Evaluation of serum and pleural levels tumor M2-pyruvate kinase in lung cancer patients with pleural effusion
Tiantian Z, et al.
BMC Pulmonary Medicine, 22, 307-307 (2022)
Development of Novel Irreversible Pyruvate Kinase M2 Inhibitors
I-Shan H, et al.
Journal of Medicinal Chemistry, 62(18), 8497-8510 (2019)
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