SRP0300
TGF-β1 Latent human
recombinant, expressed in FreeStyle™ 293-F cells, ≥98% (SDS-PAGE)
别名:
LAP, TGFb1, TGFbeta1, latency-associated peptide, transforming growth factor beta-1
biological source
human
recombinant
expressed in FreeStyle™ 293-F cells
assay
≥98% (SDS-PAGE)
form
aqueous solution
mol wt
25 kDa
packaging
pkg of 5 μg
manufacturer/tradename
Invitrogen
storage condition
avoid repeated freeze/thaw cycles
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... LTBP1(4052)
General description
Human TGFβ1 (GenBank Accession No. NM_000660), amino acids 30-390 (end) with Cys33Ser mutation and N-terminal His-tag, disulfide-linked homodimers of TGFβ1 LAP and mature TGFβ1, MW= ~75kDa and 25kDa, under non-reducing conditions.
LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3.
LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3.
Application
Useful for cell culture and for the study of signaling and apoptotic pathways.
Biochem/physiol Actions
LTBP1 (latent transforming growth factor β binding protein 1) is an extracellular matrix protein which also controls TGF-β (transforming growth factor β) bioavailability. LTBP1 is mainly responsible for the folding and secretion of TGF-β, thereby adding it to the extracellular matrix. It is a target of HTRA1 (high temperature requirement protein A1), a serine protease involved in cellular signaling.
Physical form
Lyophilized from a 0.2 μM filtered, aqueous solution.
Preparation Note
Reconstitute in sterile PBS with 0.1% BSA. Stability: >6 months at –70°C (as a solid). Store reconstituted protein in aliquots at –80°C for up to 3 months. Avoid freeze/thaw cycles.
Legal Information
FreeStyle is a trademark of Invitrogen Corp.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Cerebral small vessel disease-related protease HtrA1 processes latent TGF-? binding protein 1 and facilitates TGF-? signaling.
Beaufort N, et al.
Proceedings of the National Academy of Sciences of the USA, 111, 16496-16501 (2014)
Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1.
Saharinen J, et al.
The Embo Journal, 15, 245-253 (1996)
A suite of novel human spermatozoal RNAs.
Ostermeier GC, et al.
Journal of Andrology, 26, 70-74 (2005)
J S Munger et al.
Kidney international, 51(5), 1376-1382 (1997-05-01)
Transforming growth factor-beta are cytokines with a wide range of biological effects. They play a pathologic role in inflammatory and fibrosing diseases such as nephrosclerosis. TGF-beta s are secreted in a latent form due to noncovalent association with latency associated
L Bourdrel et al.
Protein expression and purification, 4(2), 130-140 (1993-04-01)
A partial cDNA clone encoding the gene for human transforming growth factor-beta 1 (TGF-beta 1) was isolated from a human bladder carcinoma cell line (5637) cDNA library. Following restriction enzyme processing and ligation of synthetic oligonucleotide linkers, the gene was
实验方案
Discover an expert protocol for seeding and culturing midbrain dopaminergic neurons in monoculture. Explore high purity cells and culture reagents.
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