生物来源
human
重组
expressed in FreeStyle™ 293-F cells
检测方案
≥90% (SDS-PAGE)
形式
aqueous solution
比活
≥3900 pmol/min-μg
分子量
36 kDa
技术
inhibition assay: suitable
适用性
suitable for molecular biology
NCBI登记号
应用
life science and biopharma
运输
dry ice
储存温度
−70°C
基因信息
human ... CTSL(1514)
一般描述
人组织蛋白酶B（基因库登录号：NM_001912），氨基酸18-333，带C-末端HIS标签，MW = 36 kDa，在FreeStyle 293-F细胞中表达。
应用
活性人组织蛋白酶L可用于激活DPP1（组织蛋白酶C）。 活性人组织蛋白酶L已用于一项研究中，该研究确定N-酰基和N-磺酰恶唑烷-2，4-二酮是丝氨酸蛋白酶的假不可逆抑制剂。 活性人组织蛋白酶L也已用于研究人衰老过程中血清中组织蛋白酶B和L活性的作用。
生化/生理作用
Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome. Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment.
单位定义
一个单位定义为在37℃下每分钟裂解1 pmol底物的酶量
外形
在50 mM MES，400 mM NaCl，pH 5.0和10%甘油中配制。
制备说明
冰上解冻。 首次解冻后，短暂旋转含有酶的试管，以回收试管中的全部内容物。将酶分装成一次性使用的等分试样。 将剩余的未稀释酶分装保存于-70℃。注：酶对冻融循环非常敏感。
法律信息
FreeStyle is a trademark of Invitrogen Corp.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Jacqueline M Lankelma et al.
Life sciences, 86(7-8), 225-233 (2009-12-05)
Cathepsin L, a cysteine protease, is considered to be a potential therapeutic target in cancer treatment. Proteases are involved in the development and progression of cancer. Inhibition of activity of specific proteases may slow down cancer progression. In this review
Prohormone Thiol Protease
Vivian Y.H. Hook
Handbook of Proteolytic Enzymes (2013)
Jana Ilgová et al.
Molecular and biochemical parasitology, 235, 111248-111248 (2019-12-25)
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably
Ricardo A Orbegozo-Medina et al.
PloS one, 14(2), e0211035-e0211035 (2019-02-02)
Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host
Peter Mundel et al.
Kidney international, 77(7), 571-580 (2009-11-20)
Proteinuria is a major health-care problem that affects several hundred million people worldwide. Proteinuria is a cardinal sign and a prognostic marker of kidney disease, and also an independent risk factor for cardiovascular morbidity and mortality. Microalbuminuria is the earliest
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