Application
活性人ATP柠檬酸裂解酶可用于研究酶动力学，筛选抑制剂和选择性分析。活性人ATP柠檬酸裂解酶已用于研究中，以确定引发ACL反应的催化磷酸化的性质，并鉴定所涉及的活性位点残基。 活性人ATP柠檬酸裂解酶也已用于分析肿瘤代谢的研究中，以揭示遗传多样的人胶质母细胞瘤中的线粒体葡萄糖氧化。
Biochem/physiol Actions
ATP柠檬酸裂解酶是一种参与脂肪酸合成的酶，可从柠檬酸和CoA生成胞质乙酰辅酶A和草酰乙酸。ATP柠檬酸裂解酶通常在癌症中上调。
General description
人ATP柠檬酸裂解酶（ACLY）（GenBank登录号NM_001096），全长，带有N端GST标签，MW=147 kDa，在杆状病毒表达系统中表达。
Other Notes
一个单位定义为在37℃下将1 pmol ADP转化为ATP/min所需的酶量。
Physical form
在mM Tris-HCl（pH 8.0）、100 mM NaCl、0.05%吐温-20和10%甘油中配制。
Preparation Note
冰上解冻。 首次解冻后，短暂旋转含有酶的试管，以回收试管中的全部内容物。将酶分装成一次性使用的等分试样。 将剩余的未稀释酶分装保存于-70℃。注：酶对冻融循环非常敏感。
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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Several studies have shown that Garcinia cambogia plays an important role in the regulation of endogenous lipid biosynthesis. This effect is specially attributed to (-)-hydroxycitric acid (HCA) inhibiting the enzyme ATP-dependent citrate lyase, which catalyzes the cleavage of citrate to
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ATP citrate lyase (ACL) catalyzes an ATP-dependent biosynthetic reaction which produces acetyl-coenzyme A and oxaloacetate from citrate and coenzyme A (CoA). Studies were performed with recombinant human ACL to ascertain the nature of the catalytic phosphorylation that initiates the ACL
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